Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

Publication: Research - peer-reviewJournal article – Annual report year: 1997

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A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
Original languageEnglish
JournalApplied and Environmental Microbiology
Publication date1997
Volume63
Issue9
Pages3698-3702
ISSN0099-2240
StatePublished
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