Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures
Publication: Research - peer-review › Journal article – Annual report year: 1997
A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
| Original language | English |
|---|---|
| Journal | Applied and Environmental Microbiology |
| Publication date | 1997 |
| Volume | 63 |
| Journal number | 9 |
| Pages | 3698-3702 |
| ISSN | 0099-2240 |
| State | Published |
ID: 5344092