Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures
Publication: Research - peer-review › Journal article – Annual report year: 1997
A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
|Journal||Applied and Environmental Microbiology|