Selection of hyperadherent mutants in Pseudomonas putida biofilms

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Selection of hyperadherent mutants in Pseudomonas putida biofilms. / Yousef-Coronado, Fatima; Soriano, María Isabel; Yang, Liang; Molin, Søren; Espinosa-Urgel, Manuel.

In: Microbiology, Vol. 157, No. Pt 8, 2011, p. 2257-2265.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Yousef-Coronado, Fatima; Soriano, María Isabel; Yang, Liang; Molin, Søren; Espinosa-Urgel, Manuel / Selection of hyperadherent mutants in Pseudomonas putida biofilms.

In: Microbiology, Vol. 157, No. Pt 8, 2011, p. 2257-2265.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{2268e1fda17641de9b226ee64d232e55,
title = "Selection of hyperadherent mutants in Pseudomonas putida biofilms",
publisher = "Society for General Microbiology",
author = "Fatima Yousef-Coronado and Soriano, {María Isabel} and Liang Yang and Søren Molin and Manuel Espinosa-Urgel",
year = "2011",
doi = "10.1099/mic.0.047787-0",
volume = "157",
number = "Pt 8",
pages = "2257--2265",
journal = "Microbiology",
issn = "1350-0872",

}

RIS

TY - JOUR

T1 - Selection of hyperadherent mutants in Pseudomonas putida biofilms

A1 - Yousef-Coronado,Fatima

A1 - Soriano,María Isabel

A1 - Yang,Liang

A1 - Molin,Søren

A1 - Espinosa-Urgel,Manuel

AU - Yousef-Coronado,Fatima

AU - Soriano,María Isabel

AU - Yang,Liang

AU - Molin,Søren

AU - Espinosa-Urgel,Manuel

PB - Society for General Microbiology

PY - 2011

Y1 - 2011

N2 - A number of genetic determinants required for bacterial colonization of solid surfaces and biofilm formation have been identified in different micro-organisms. There are fewer accounts of mutations that favour the transition to a sessile mode of life. Here we report the isolation of random transposon Pseudomonas putida KT2440 mutants showing increased biofilm formation, and the detailed characterization of one of them. This mutant exhibits a complex phenotype, including altered colony morphology, increased production of extracellular polymeric substances and enhanced swarming motility, along with the formation of denser and more complex biofilms than the parental strain. Sequence analysis revealed that the pleiotropic phenotype exhibited by the mutant resulted from the accumulation of two mutations: a transposon insertion, which disrupted a predicted outer membrane lipoprotein, and a point mutation in lapG, a gene involved in the turnover of the large adhesin LapA. The contribution of each alteration to the phenotype and the possibility that prolonged sessile growth results in the selection of hyperadherent mutants are discussed.

AB - A number of genetic determinants required for bacterial colonization of solid surfaces and biofilm formation have been identified in different micro-organisms. There are fewer accounts of mutations that favour the transition to a sessile mode of life. Here we report the isolation of random transposon Pseudomonas putida KT2440 mutants showing increased biofilm formation, and the detailed characterization of one of them. This mutant exhibits a complex phenotype, including altered colony morphology, increased production of extracellular polymeric substances and enhanced swarming motility, along with the formation of denser and more complex biofilms than the parental strain. Sequence analysis revealed that the pleiotropic phenotype exhibited by the mutant resulted from the accumulation of two mutations: a transposon insertion, which disrupted a predicted outer membrane lipoprotein, and a point mutation in lapG, a gene involved in the turnover of the large adhesin LapA. The contribution of each alteration to the phenotype and the possibility that prolonged sessile growth results in the selection of hyperadherent mutants are discussed.

U2 - 10.1099/mic.0.047787-0

DO - 10.1099/mic.0.047787-0

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - Pt 8

VL - 157

SP - 2257

EP - 2265

ER -