Screening the natural habitat: New peptaibiotics from specimens and pure cultures of the fungicolous fungus Hypocrea pulvinata

Publication: Research - peer-reviewConference abstract in journal – Annual report year: 2012

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In order to further investigate the significance of antibiotics for the producing organism(s) in the natural habitat, we screened specimens of the fungicolous fungus Hypocrea pulvinata growing on its natural hosts Piptoporus betulinus and Fomitopsis pinicola1. Using a peptaibiomics approach2, we detected 17-, 18-, 19- (major sequences), and 20-residue peptide sequences in the five specimens
analyzed by (U)HPLC/HR-ESI-QqTOF-MS. Structures of peptaibiotics found were independently confirmed by analyzing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the specimens1. Major, 19-residue peptaibols were assigned as deletion sequences of the trichosporins B3 lacking the Ala/Aib residue in position3. Our results corroborate that: i) peptaibiotics are, indeed, biosynthesized in the natural habitat, thus, ii) their membrane-perturbing formation of ion channels may support the parasitic life style of a fungicolous fungus.
Original languageEnglish
JournalJournal of Peptide Science
Publication date2012
Volume18
IssueS1
PagesS74-S74
ISSN1075-2617
StatePublished

Conference

Conference32nd EPS Meeting
CountryGreece
CityAthens
Period02/09/1207/09/12

Bibliographical note

Poster presentation

Keywords

  • BIOCHEMISTRY, CHEMISTRY
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