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We introduce a new method of rotational image acquisition for four dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional (3D) data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating HH stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition. The result is validated by comparison of calculated and orignal B-scan sequences (see Figure).
Original languageEnglish
JournalCLINICAL RESEARCH IN CARDIOLOGY
Volume100
Issue number9
Pages (from-to)834
ISSN1861-0684
DOIs
StatePublished - 2011
Peer-reviewedYes

Conference

ConferenceJahrestagung der Deutschen Gesellschaft für Pädiatrische Kardiologie
Number43
CityWeimar, Germany
Period01/01/2011 → …
CitationsWeb of Science® Times Cited: No match on DOI
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