Reconstruction of the central carbon metabolism of Aspergillus niger

Publication: Research - peer-reviewJournal article – Annual report year: 2003

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Reconstruction of the central carbon metabolism of Aspergillus niger. / David, Helga; Åkesson, Mats Fredrik; Nielsen, Jens.

In: European journal of biochemistry, Vol. 270, No. 21, 2003, p. 4243-4253.

Publication: Research - peer-reviewJournal article – Annual report year: 2003

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David, Helga; Åkesson, Mats Fredrik; Nielsen, Jens / Reconstruction of the central carbon metabolism of Aspergillus niger.

In: European journal of biochemistry, Vol. 270, No. 21, 2003, p. 4243-4253.

Publication: Research - peer-reviewJournal article – Annual report year: 2003

Bibtex

@article{8142aeab168c4202b34ee53b8206409c,
title = "Reconstruction of the central carbon metabolism of Aspergillus niger",
publisher = "Springer Verlag",
author = "Helga David and Åkesson, {Mats Fredrik} and Jens Nielsen",
year = "2003",
volume = "270",
number = "21",
pages = "4243--4253",
journal = "European journal of biochemistry",
issn = "0014-2956",

}

RIS

TY - JOUR

T1 - Reconstruction of the central carbon metabolism of Aspergillus niger

A1 - David,Helga

A1 - Åkesson,Mats Fredrik

A1 - Nielsen,Jens

AU - David,Helga

AU - Åkesson,Mats Fredrik

AU - Nielsen,Jens

PB - Springer Verlag

PY - 2003

Y1 - 2003

N2 - The topology of central carbon metabolism of Aspergillus niger was identified and the metabolic network reconstructed, by integrating genomic, biochemical and physiological information available for this microorganism and other related fungi. The reconstructed network may serve as a valuable database for annotation of genes identified in future genome sequencing projects on aspergilli. Based on the metabolic reconstruction, a stoichiometric model was set up that includes 284 metabolites and 335 reactions, of which 268 represent biochemical conversions and 67 represent transport processes between the different intracellular compartments and between the cell and the extracellular medium. The stoichiometry of the metabolic reactions was used in combination with biosynthetic requirements for growth and pseudo-steady state mass balances over intracellular metabolites for the quanti. cation of metabolic fluxes using metabolite balancing. This framework was employed to perform an in silico characterisation of the phenotypic behaviour of A. niger grown on different carbon sources. The effects on growth of single reaction deletions were assessed and essential biochemical reactions were identified for different carbon sources. Furthermore, application of the stoichiometric model for assessing the metabolic capabilities of A. niger to produce metabolites was evaluated by using succinate production as a case study.

AB - The topology of central carbon metabolism of Aspergillus niger was identified and the metabolic network reconstructed, by integrating genomic, biochemical and physiological information available for this microorganism and other related fungi. The reconstructed network may serve as a valuable database for annotation of genes identified in future genome sequencing projects on aspergilli. Based on the metabolic reconstruction, a stoichiometric model was set up that includes 284 metabolites and 335 reactions, of which 268 represent biochemical conversions and 67 represent transport processes between the different intracellular compartments and between the cell and the extracellular medium. The stoichiometry of the metabolic reactions was used in combination with biosynthetic requirements for growth and pseudo-steady state mass balances over intracellular metabolites for the quanti. cation of metabolic fluxes using metabolite balancing. This framework was employed to perform an in silico characterisation of the phenotypic behaviour of A. niger grown on different carbon sources. The effects on growth of single reaction deletions were assessed and essential biochemical reactions were identified for different carbon sources. Furthermore, application of the stoichiometric model for assessing the metabolic capabilities of A. niger to produce metabolites was evaluated by using succinate production as a case study.

JO - European journal of biochemistry

JF - European journal of biochemistry

SN - 0014-2956

IS - 21

VL - 270

SP - 4243

EP - 4253

ER -