Reconciliation of opposing views on membrane-sugar interactions.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Reconciliation of opposing views on membrane-sugar interactions.. / Andersen, Heidi D.; Wang, Chunhua; Arleth, Lise; Peters, Günther H.J.; Westh, Peter.

In: National Academy of Sciences. Proceedings, Vol. 108, No. 5, 2011, p. 1874-1878.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Andersen, Heidi D.; Wang, Chunhua; Arleth, Lise; Peters, Günther H.J.; Westh, Peter / Reconciliation of opposing views on membrane-sugar interactions..

In: National Academy of Sciences. Proceedings, Vol. 108, No. 5, 2011, p. 1874-1878.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{5c991b9ba4c844ae9a03bd37f97b6f03,
title = "Reconciliation of opposing views on membrane-sugar interactions.",
publisher = "National Academy of Sciences",
author = "Andersen, {Heidi D.} and Chunhua Wang and Lise Arleth and Peters, {Günther H.J.} and Peter Westh",
year = "2011",
doi = "10.1073/pnas.1012516108",
volume = "108",
number = "5",
pages = "1874--1878",
journal = "National Academy of Sciences. Proceedings",
issn = "0027-8424",

}

RIS

TY - JOUR

T1 - Reconciliation of opposing views on membrane-sugar interactions.

A1 - Andersen,Heidi D.

A1 - Wang,Chunhua

A1 - Arleth,Lise

A1 - Peters,Günther H.J.

A1 - Westh,Peter

AU - Andersen,Heidi D.

AU - Wang,Chunhua

AU - Arleth,Lise

AU - Peters,Günther H.J.

AU - Westh,Peter

PB - National Academy of Sciences

PY - 2011

Y1 - 2011

N2 - It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above â¼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar-membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.

AB - It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above â¼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar-membrane interactions offers a reconciliation of conflicting views in earlier reports on sugar-induced modulations of membrane properties.

KW - Preferential binding

KW - Membrane interface

KW - Interaction free energy

KW - Membrane structure

KW - Preferential exclusion

U2 - 10.1073/pnas.1012516108

DO - 10.1073/pnas.1012516108

JO - National Academy of Sciences. Proceedings

JF - National Academy of Sciences. Proceedings

SN - 0027-8424

IS - 5

VL - 108

SP - 1874

EP - 1878

ER -