Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Research output: Research - peer-reviewJournal article – Annual report year: 2013

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The suitable optical properties of thiol–ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol–ene chips. This work focuses on thiol–ene substrates featuring an excess of thiol groups at their surface. The thiol–ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ! 17 SH nm"2. Biotin alkyne was patterned directly inside thiol–ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent waveinduced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.
Original languageEnglish
JournalAnalyst
Volume138
Issue number3
Pages (from-to)845-849
ISSN0003-2654
DOIs
StatePublished - 2013
CitationsWeb of Science® Times Cited: 26

    Research areas

  • Thiol-ene polymers, Biomolecule immobilization, Surface functionalization, Biochips
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