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Rainbow trout surviving infections of viral haemorrhagic septicemia virus (VHSV) show lasting antibodies to recombinant G protein fragments. / Encinas, P.; Gomez-Casado, E.; Grandes, Fregeneda; Olesen, Niels Jørgen; Lorenzen, Niels; Estepa, A.; Coll, J. M.

In: Fish and Shellfish Immunology, Vol. 30, No. 3, 2011, p. 929-935.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Encinas, P.; Gomez-Casado, E.; Grandes, Fregeneda; Olesen, Niels Jørgen; Lorenzen, Niels; Estepa, A.; Coll, J. M. / Rainbow trout surviving infections of viral haemorrhagic septicemia virus (VHSV) show lasting antibodies to recombinant G protein fragments.

In: Fish and Shellfish Immunology, Vol. 30, No. 3, 2011, p. 929-935.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{31df6de10b2d40e9b8b73ea5e4e32d38,
title = "Rainbow trout surviving infections of viral haemorrhagic septicemia virus (VHSV) show lasting antibodies to recombinant G protein fragments",
publisher = "Academic Press",
author = "P. Encinas and E. Gomez-Casado and Fregeneda Grandes and Olesen, {Niels Jørgen} and Niels Lorenzen and A. Estepa and Coll, {J. M.}",
year = "2011",
doi = "10.1016/j.fsi.2011.01.021",
volume = "30",
number = "3",
pages = "929--935",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",

}

RIS

TY - JOUR

T1 - Rainbow trout surviving infections of viral haemorrhagic septicemia virus (VHSV) show lasting antibodies to recombinant G protein fragments

A1 - Encinas,P.

A1 - Gomez-Casado,E.

A1 - Grandes,Fregeneda

A1 - Olesen,Niels Jørgen

A1 - Lorenzen,Niels

A1 - Estepa,A.

A1 - Coll,J. M.

AU - Encinas,P.

AU - Gomez-Casado,E.

AU - Grandes,Fregeneda

AU - Olesen,Niels Jørgen

AU - Lorenzen,Niels

AU - Estepa,A.

AU - Coll,J. M.

PB - Academic Press

PY - 2011

Y1 - 2011

N2 - Rainbow trout antibodies (Abs) binding to recombinant fragments (frgs) derived from the protein G of the viral haemorrhagic septicemia virus (VHSV)-07.71 strain, could be detected by ELISA (frg-ELISA) in sera from trout surviving laboratory-controlled infections. Abs were detected not only by using sera from trout infected with the homologous VHSV isolate but also with the VHSV-DK-201433 heterologous isolate, which had 13 amino acid changes. Sera from healthy trout and/or from trout surviving infectious haematopoietic necrosis virus (IHNV) infection, were used to calculate cut-off absorbances to differentiate negative from positive sera. Specific anti-VHSV Abs could then be detected by using any of the following frgs: frg11 (56–110), frg15 (65–250), frg16 (252–450) or G21-465. While high correlations were found among the ELISA values obtained with the different frgs, no correlations between any frg-ELISA and complement-dependent 50% plaque neutralization test (PNT) titres could be demonstrated. Between 4 and 10 weeks after VHSV infection, more trout sera were detected as positives by using heterologous frg-ELISA rather than homologous PNT. Furthermore, the percentage of positive sera detected by frg11-ELISA increased with time after infection to reach 100%, while those detected by complement-dependent PNT decreased to 29.4%, thus confirming that the lack of neutralizing Abs does not mean the lack of any anti-VHSV Abs in survivor trout sera. Preliminary results with sera from field samples suggest that further refinements of the frg-ELISA could allow detection of anti-VHSV trout Abs in natural outbreaks caused by different heterologous VHSV isolates. The homologous frg-ELISA method could be useful to follow G immunization attempts during vaccine development and/or to best understand the fish Ab response during VHSV infections. The viral frgs approach might also be used with other fish species and/or viruses.

AB - Rainbow trout antibodies (Abs) binding to recombinant fragments (frgs) derived from the protein G of the viral haemorrhagic septicemia virus (VHSV)-07.71 strain, could be detected by ELISA (frg-ELISA) in sera from trout surviving laboratory-controlled infections. Abs were detected not only by using sera from trout infected with the homologous VHSV isolate but also with the VHSV-DK-201433 heterologous isolate, which had 13 amino acid changes. Sera from healthy trout and/or from trout surviving infectious haematopoietic necrosis virus (IHNV) infection, were used to calculate cut-off absorbances to differentiate negative from positive sera. Specific anti-VHSV Abs could then be detected by using any of the following frgs: frg11 (56–110), frg15 (65–250), frg16 (252–450) or G21-465. While high correlations were found among the ELISA values obtained with the different frgs, no correlations between any frg-ELISA and complement-dependent 50% plaque neutralization test (PNT) titres could be demonstrated. Between 4 and 10 weeks after VHSV infection, more trout sera were detected as positives by using heterologous frg-ELISA rather than homologous PNT. Furthermore, the percentage of positive sera detected by frg11-ELISA increased with time after infection to reach 100%, while those detected by complement-dependent PNT decreased to 29.4%, thus confirming that the lack of neutralizing Abs does not mean the lack of any anti-VHSV Abs in survivor trout sera. Preliminary results with sera from field samples suggest that further refinements of the frg-ELISA could allow detection of anti-VHSV trout Abs in natural outbreaks caused by different heterologous VHSV isolates. The homologous frg-ELISA method could be useful to follow G immunization attempts during vaccine development and/or to best understand the fish Ab response during VHSV infections. The viral frgs approach might also be used with other fish species and/or viruses.

KW - VHSV

KW - Fragments

KW - Viral haemorrhagic septicemia

KW - Trout antibodies

KW - G protein

U2 - 10.1016/j.fsi.2011.01.021

DO - 10.1016/j.fsi.2011.01.021

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 3

VL - 30

SP - 929

EP - 935

ER -