Quantitative pupil analysis in stimulated emission depletion microscopy using phase retrieval.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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  • Author: Kromann, Emil B

    Department of Cell Biology, Yale University School of Medicine, United States

  • Author: Gould, Travis J

    Department of Cell Biology, Yale University School of Medicine, United States

  • Author: Juette, Manuel F

    Department of Cell Biology, Yale University School of Medicine, United States

  • Author: Wilhjelm, Jens E

    Biomedical Engineering, Department of Electrical Engineering, Technical University of Denmark, Ørsteds Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Bewersdorf, Joerg

    Department of Biomedical Engineering, Yale University School of Medicine, United States

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The resolution attainable with stimulated emission depletion (STED) microscopy greatly depends on the quality of the STED laser focus. So far, visual inspection of a measured STED focus has been the only convenient means of gauging the source of aberrations. Here we describe a method, requiring no instrument modifications, for obtaining an equivalent to the complex pupil function at the back aperture of the objective and show that it provides quantitative information about aberration sources (including aberrations induced by the objective or sample). We show the accuracy of this field representation to be sufficient for reconstructing the STED focus in three dimensions and determining corrective steps.
Original languageEnglish
JournalOptics Letters
Publication date2012
Volume37
Issue11
Pages1805-1807
ISSN0146-9592
StatePublished
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