• Author: Gieseker, Charles M.

    Food and Drug Administration, Center for Veterinary Medicine, United States

  • Author: Mayer, Tamara D.

    Food and Drug Administration, Center for Veterinary Medicine, United States

  • Author: Crosby, Tina C.

    Food and Drug Administration, Center for Veterinary Medicine, United States

  • Author: Carson, Jeremy

    Fish Health Unit, Department of Primary Industries, Parks, Water & Environment, Australia

  • Author: Dalsgaard, Inger

    Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870, Frederiksberg C, Denmark

  • Author: Darwish, Ahmed M.

    United States Department of Agriculture, United States

  • Author: Gaunt, Patricia S.

    Mississippi State University, United States

  • Author: Gao, Dana X.

    Mississippi State University, United States

  • Author: Hsu, Hui-Min

    University of Wisconsin, United States

  • Author: Lin, Tsang L.

    Purdue University, United States

  • Author: Oaks, J. Lindsay

    University of Washington, United States

  • Author: Pyecroft, Melissa

    Fish Health Unit, Department of Primary Industries, Parks, Water & Environment, Australia

  • Author: Teitzel, Charlene

    University of Washington, United States

  • Author: Somsiri, Temdoung

    Kasetsart University, Thailand

  • Author: Wu, Ching C.

    Purdue University, United States

View graph of relations

A multi-laboratory broth microdilution method trial was performed to standardize the specialized test conditions required for the fish pathogens Flavobacterium columnare and F. psychrophilum. Nine laboratories tested the quality control (QC) strains Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 against 10 antimicrobials (ampicillin, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, ormetoprim/sulfadimethoxine, oxolinic acid, oxytetracycline, and trimethoprim/sulfamethoxazole) in diluted (4 g l−1) cation-adjusted Mueller-Hinton broth incubated at 28 and 18°C for 44–48 and 92–96 h, respectively. QC ranges were set for 9 of the 10 antimicrobials. Most of the minimal inhibitory concentration (MIC) distributions (16 of 18, 9 drugs at both temperatures) for A. salmonicida ATCC 33658 were centered on a single median MIC ± 1 two-fold drug dilution resulting in a QC range that spanned 3 dilutions. More of the E. coli ATCC 25922 MIC distributions (7 of 16) were centered between 2 MIC dilutions requiring a QC range that spanned 4 dilutions. A QC range could not be determined for E. coli ATCC 25922 against 2 antimicrobials at the low temperature. These data and their associated QC ranges have been approved by the Clinical and Laboratory Standards Institute (CLSI), and will be included in the next edition of the CLSI M49-A Guideline. This method represents the first standardized reference method for testing fish pathogenic Flavobacterium spp.
Original languageEnglish
JournalDiseases of Aquatic Organisms
Publication date2012
Volume101
Issue3
Pages207-215
ISSN0177-5103
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 0

Keywords

  • Standard methods, Antimicrobial susceptibility testing, Fish, Flavobacteria
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 33438047