• Author: Husain, Mainul

    Health Canada, Canada

  • Author: Saber, Anne Thoustrup

    National Research Center for Working Environment, Denmark

  • Author: Guo, Charles

    Health Canada, Canada

  • Author: Jacobsen, Nicklas R.

    National Research Center for Working Environment, Denmark

  • Author: Jensen, Keld Almand

    National Research Center for Working Environment, Denmark

  • Author: Yauk, Carole L.

    Health Canada, Canada

  • Author: Williams, Andrew Richard

    National Research Center for Working Environment, Denmark

  • Author: Vogel, Ulla Birgitte

    Department of Micro- and Nanotechnology, Technical University of Denmark

  • Author: Wallin, Hakan

    National Research Center for Working Environment, Denmark

  • Author: Halappanavar, Sabina

    Health Canada, Canada

View graph of relations

We investigated gene expression, protein synthesis, and particle retention in mouse lungs following intratracheal instillation of varying doses of nano-sized titanium dioxide (nano-TiO2). Female C57BL/6 mice were exposed to rutile nano-TiO2 via single intratracheal instillations of 18, 54, and 162μg/mouse. Mice were sampled 1, 3, and 28days post-exposure. The deposition of nano-TiO2 in the lungs was assessed using nanoscale hyperspectral microscopy. Biological responses in the pulmonary system were analyzed using DNA microarrays, pathway-specific real-time RT-PCR (qPCR), gene-specific qPCR arrays, and tissue protein ELISA. Hyperspectral mapping showed dose-dependent retention of nano-TiO2 in the lungs up to 28days post-instillation. DNA microarray analysis revealed approximately 3000 genes that were altered across all treatment groups (±1.3 fold; p
Original languageEnglish
JournalToxicology and Applied Pharmacology
Volume269
Issue number3
Pages (from-to)250-262
ISSN0041-008X
DOIs
StatePublished - 2013
CitationsWeb of Science® Times Cited: 48

    Keywords

  • Hyperspectral microscopy, Gene expression, Inflammation, Muscle contraction, Calcium homeostasis, Tissue particle retention
Download as:
Download as PDF
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
Word

Download statistics

No data available

ID: 55912326