TY - JOUR

T1 - Proteome Analysis of Pyloric Ceca: A Methodology for Fish Feed Development?

A1 - Wulff,Tune

A1 - Petersen,Jørgen

A1 - Nørrelykke,Mette R.

A1 - Jessen,Flemming

A1 - Nielsen,Henrik H.

AU - Wulff,Tune

AU - Petersen,Jørgen

AU - Nørrelykke,Mette R.

AU - Jessen,Flemming

AU - Nielsen,Henrik H.

PB - American Chemical Society

PY - 2012

Y1 - 2012

N2 - Changing the protein source of fish feed from fish meal to alternative sources of protein will affect traits such as fish growth, quality, and feed utilization. The present investigation was initiated to introduce a two-dimensional gel electrophoresis based proteomic workflow as a tool to investigate feed effects on fish by analyzing protein changes in the fish gut. The workflow was used to study the effect of substituting fish meal in fish feed by alternative sources of protein. Rainbow trout divided into five groups were fed for 72 days with feeds varying in protein composition. By two-dimensional gel electrophoresis proteins extracted from the pyloric ceca were separated, making it possible to measure the abundance of more than 440 protein spots. The expression of 41 protein spots was found to change due to differences in feed composition. By mass spectrometry 31 of these proteins were identified, including proteins involved in digestion (trypsinogen, carboxylic ester hydrolase, and aminopeptidase). The many expression changes indicated that the trout, when adapting to differences in feed formulation, alter the protein composition of the gut.

AB - Changing the protein source of fish feed from fish meal to alternative sources of protein will affect traits such as fish growth, quality, and feed utilization. The present investigation was initiated to introduce a two-dimensional gel electrophoresis based proteomic workflow as a tool to investigate feed effects on fish by analyzing protein changes in the fish gut. The workflow was used to study the effect of substituting fish meal in fish feed by alternative sources of protein. Rainbow trout divided into five groups were fed for 72 days with feeds varying in protein composition. By two-dimensional gel electrophoresis proteins extracted from the pyloric ceca were separated, making it possible to measure the abundance of more than 440 protein spots. The expression of 41 protein spots was found to change due to differences in feed composition. By mass spectrometry 31 of these proteins were identified, including proteins involved in digestion (trypsinogen, carboxylic ester hydrolase, and aminopeptidase). The many expression changes indicated that the trout, when adapting to differences in feed formulation, alter the protein composition of the gut.

KW - feed utilization

KW - fish feed

KW - Oncorhynchus mykiss

KW - proteomic

KW - pyloric ceca

KW - two-dimensional gel electrophoresis

U2 - 10.1021/jf3016943

DO - 10.1021/jf3016943

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 34

VL - 60

SP - 8457

EP - 8464

ER -