Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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  • Author: Podolska, Agnieszka, Denmark

    Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Denmark

  • Author: Anthon, Christian, Denmark

    Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Denmark

  • Author: Bak, Mads, Denmark

    Center for non-coding RNA in Technology and Health, University of Copenhagen, Denmark

  • Author: Tommerup, Niels, Denmark

    Center for non-coding RNA in Technology and Health, University of Copenhagen, Denmark

  • Author: Skovgaard, Kerstin

    Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Heegaard, Peter M. H.

    Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Gorodkin, Jan, Denmark

    Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Denmark

  • Author: Cirera, Susanna, Denmark

    Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Denmark

  • Author: Fredholm, Merete, Denmark

    Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Denmark

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Background: MicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus pleuropneumoniae (APP) causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is still very limited.

Results: In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were significantly up-regulated in the necrotic sample and 12 were down-regulated. The expression analysis of a number of candidates revealed microRNAs of potential importance in the innate immune response. MiR-155, a known key player in inflammation, was found expressed in both samples. Moreover, miR-664-5p, miR-451 and miR-15a appear as very promising candidates for microRNAs involved in response to pathogen infection.

Conclusions: This is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.
Original languageEnglish
JournalB M C Genomics
Publication date2012
Volume13
Pages459
ISSN1471-2164
DOIs
StatePublished

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

CitationsWeb of Science® Times Cited: 3

Keywords

  • MicroRNA, RNAseq, High throughput sequencing, RT-qPCR, Actinobacillus pleuropneumoniae, Pig, Pleuropneumonia
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