Production of Metabolites
Publication: Research › Patent – Annual report year: 2012
A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
| Original language | English |
|---|---|
| Publication date | 2011 |
| Patent number | WO2011147818 |
| Date | 01-12-11 |
| Country | International Bureau of the World Intellectual Property Organization (WIPO) |
| State | Published |
ID: 12598234