Production of 2nd generation Bioethanol from Lucerne - Optimization of Hydrothermal Pretreatment

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205 degrees C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.
Original languageEnglish
JournalBioResources
Publication date2012
Volume7
Journal number2
Pages1582-1593
ISSN1930-2126
StatePublished

Keywords

  • MATERIALS, WET OXIDATION PRETREATMENT, DILUTE-ACID PRETREATMENT, WHEAT-STRAW, ETHANOL-PRODUCTION, SUGARCANE BAGASSE, ENZYMATIC-HYDROLYSIS, STEAM EXPLOSION, ALFALFA, FERMENTATION, CELLULOSE
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