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Probing the redox metabolism in the strictly anaerobic, extremely thermophilic, hydrogen-producing Caldicellulosiruptor saccharolyticus using amperometry. / Kostesha, Natalie; Willquist, Karin; Emnéus, Jenny; Niel, Ed W. J. van.

In: Extremophiles, Vol. 15, No. 1, 2011, p. 77-87.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Kostesha, Natalie; Willquist, Karin; Emnéus, Jenny; Niel, Ed W. J. van / Probing the redox metabolism in the strictly anaerobic, extremely thermophilic, hydrogen-producing Caldicellulosiruptor saccharolyticus using amperometry.

In: Extremophiles, Vol. 15, No. 1, 2011, p. 77-87.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{88e211e7ce88477eb2718de46b465435,
title = "Probing the redox metabolism in the strictly anaerobic, extremely thermophilic, hydrogen-producing Caldicellulosiruptor saccharolyticus using amperometry",
publisher = "Springer Japan KK",
author = "Natalie Kostesha and Karin Willquist and Jenny Emnéus and Niel, {Ed W. J. van}",
year = "2011",
doi = "10.1007/s00792-010-0341-4",
volume = "15",
number = "1",
pages = "77--87",
journal = "Extremophiles",
issn = "1431-0651",

}

RIS

TY - JOUR

T1 - Probing the redox metabolism in the strictly anaerobic, extremely thermophilic, hydrogen-producing Caldicellulosiruptor saccharolyticus using amperometry

A1 - Kostesha,Natalie

A1 - Willquist,Karin

A1 - Emnéus,Jenny

A1 - Niel,Ed W. J. van

AU - Kostesha,Natalie

AU - Willquist,Karin

AU - Emnéus,Jenny

AU - Niel,Ed W. J. van

PB - Springer Japan KK

PY - 2011

Y1 - 2011

N2 - Changes in the redox metabolism in the anaerobic, extremely thermophilic, hydrogen-forming bacterium Caldicellulosiruptor saccharolyticus were probed for the first time in vivo using mediated amperometry with ferricyanide as a thermotolerant external mediator. Clear differences in the intracellular electron flow were observed when cells were supplied with different carbon sources. A higher electrochemical response was detected when cells were supplied with xylose than with sucrose or glucose. Moreover, using the mediated electrochemical method, it was possible to detect differences in the electron flow between cells harvested in the exponential and stationary growth phases. The electron flow of C. saccharolyticus was dependent on the NADH- and reduced ferredoxin generation flux and the competitive behavior of cytosolic and membrane-associated oxidoreductases. Sodium oxamate was used to inhibit the NADH-dependent lactate dehydrogenase, upon which more NADH was directed to membrane-associated enzymes for ferricyanide reduction, leading to a higher electrochemical signal. The method is noninvasive and the results presented here demonstrate that this method can be used to accurately detect changes in the intracellular electron flow and to probe redox enzyme properties of a strictly anaerobic thermophile in vivo.

AB - Changes in the redox metabolism in the anaerobic, extremely thermophilic, hydrogen-forming bacterium Caldicellulosiruptor saccharolyticus were probed for the first time in vivo using mediated amperometry with ferricyanide as a thermotolerant external mediator. Clear differences in the intracellular electron flow were observed when cells were supplied with different carbon sources. A higher electrochemical response was detected when cells were supplied with xylose than with sucrose or glucose. Moreover, using the mediated electrochemical method, it was possible to detect differences in the electron flow between cells harvested in the exponential and stationary growth phases. The electron flow of C. saccharolyticus was dependent on the NADH- and reduced ferredoxin generation flux and the competitive behavior of cytosolic and membrane-associated oxidoreductases. Sodium oxamate was used to inhibit the NADH-dependent lactate dehydrogenase, upon which more NADH was directed to membrane-associated enzymes for ferricyanide reduction, leading to a higher electrochemical signal. The method is noninvasive and the results presented here demonstrate that this method can be used to accurately detect changes in the intracellular electron flow and to probe redox enzyme properties of a strictly anaerobic thermophile in vivo.

U2 - 10.1007/s00792-010-0341-4

DO - 10.1007/s00792-010-0341-4

JO - Extremophiles

JF - Extremophiles

SN - 1431-0651

IS - 1

VL - 15

SP - 77

EP - 87

ER -