Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape. / Liu, Liyu; Sun, Bo; Pedersen, Jonas Nyvold; Yong, Koh-Meng Aw; Getzenberg, Robert H.; Stone, Howard A.; Austin, Robert H.

In: National Academy of Sciences. Proceedings, 2011.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Liu, Liyu; Sun, Bo; Pedersen, Jonas Nyvold; Yong, Koh-Meng Aw; Getzenberg, Robert H.; Stone, Howard A.; Austin, Robert H. / Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape.

In: National Academy of Sciences. Proceedings, 2011.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{c6b1814a07f0439ab17e6d37541fe8f7,
title = "Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape",
publisher = "National Academy of Sciences",
author = "Liyu Liu and Bo Sun and Pedersen, {Jonas Nyvold} and Yong, {Koh-Meng Aw} and Getzenberg, {Robert H.} and Stone, {Howard A.} and Austin, {Robert H.}",
year = "2011",
doi = "10.1073/pnas.1102808108",
journal = "National Academy of Sciences. Proceedings",
issn = "0027-8424",

}

RIS

TY - JOUR

T1 - Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape

A1 - Liu,Liyu

A1 - Sun,Bo

A1 - Pedersen,Jonas Nyvold

A1 - Yong,Koh-Meng Aw

A1 - Getzenberg,Robert H.

A1 - Stone,Howard A.

A1 - Austin,Robert H.

AU - Liu,Liyu

AU - Sun,Bo

AU - Pedersen,Jonas Nyvold

AU - Yong,Koh-Meng Aw

AU - Getzenberg,Robert H.

AU - Stone,Howard A.

AU - Austin,Robert H.

PB - National Academy of Sciences

PY - 2011

Y1 - 2011

N2 - The metastatic invasion of cancer cells from primary tumors to distant ecological niches, rather than the primary tumors, is the cause of much cancer mortality [Zhang QB, et al. (2010) Int J Cancer 126:2534–2541; Chambers AF, Goss PE (2008) Breast Cancer Res 10:114]. Metastasis is a three-dimensional invasion process where cells spread from their site of origin and colonize distant microenvironmental niches. It is critical to be able to assess quantitatively the metastatic potential of cancer cells [Harma V, et al. (2010) PLoS ONE 5:e10431]. We have constructed a microfabricated chip with a three-dimensional topology consisting of lowlands and isolated square highlands (Tepuis), which stand hundreds of microns above the lowlands, in order to assess cancer cell metastatic potential as they invade the highlands. As a test case, the invasive ascents of the Tepui by highly metastatic PC-3 and noninvasive LNCaP prostate cancer cells were used. The vertical ascent by prostate cancer cells from the lowlands to the tops of the Tepui was imaged using confocal microscopy and used as a measure of the relative invasiveness. The less-metastatic cells (LNCaP) never populated all available tops, leaving about 15% of them unoccupied, whereas the more metastatic PC-3 cells occupied all available Tepuis. We argue that this distinct difference in invasiveness is due to contact inhibition.

AB - The metastatic invasion of cancer cells from primary tumors to distant ecological niches, rather than the primary tumors, is the cause of much cancer mortality [Zhang QB, et al. (2010) Int J Cancer 126:2534–2541; Chambers AF, Goss PE (2008) Breast Cancer Res 10:114]. Metastasis is a three-dimensional invasion process where cells spread from their site of origin and colonize distant microenvironmental niches. It is critical to be able to assess quantitatively the metastatic potential of cancer cells [Harma V, et al. (2010) PLoS ONE 5:e10431]. We have constructed a microfabricated chip with a three-dimensional topology consisting of lowlands and isolated square highlands (Tepuis), which stand hundreds of microns above the lowlands, in order to assess cancer cell metastatic potential as they invade the highlands. As a test case, the invasive ascents of the Tepui by highly metastatic PC-3 and noninvasive LNCaP prostate cancer cells were used. The vertical ascent by prostate cancer cells from the lowlands to the tops of the Tepui was imaged using confocal microscopy and used as a measure of the relative invasiveness. The less-metastatic cells (LNCaP) never populated all available tops, leaving about 15% of them unoccupied, whereas the more metastatic PC-3 cells occupied all available Tepuis. We argue that this distinct difference in invasiveness is due to contact inhibition.

U2 - 10.1073/pnas.1102808108

DO - 10.1073/pnas.1102808108

JO - National Academy of Sciences. Proceedings

JF - National Academy of Sciences. Proceedings

SN - 0027-8424

ER -