Probing the invasiveness of prostate cancer cells in a 3D microfabricated landscape

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Without internal affiliation

  • Author: Liu, Liyu

    Princeton University

  • Author: Sun, Bo

    Princeton University

  • Author: Pedersen, Jonas Nyvold


  • Author: Yong, Koh-Meng Aw

    Johns Hopkins University

  • Author: Getzenberg, Robert H.

    Johns Hopkins University

  • Author: Stone, Howard A.

    Princeton University

  • Author: Austin, Robert H.

    Princeton University

View graph of relations

The metastatic invasion of cancer cells from primary tumors to distant ecological niches, rather than the primary tumors, is the cause of much cancer mortality [Zhang QB, et al. (2010) Int J Cancer 126:2534–2541; Chambers AF, Goss PE (2008) Breast Cancer Res 10:114]. Metastasis is a three-dimensional invasion process where cells spread from their site of origin and colonize distant microenvironmental niches. It is critical to be able to assess quantitatively the metastatic potential of cancer cells [Harma V, et al. (2010) PLoS ONE 5:e10431]. We have constructed a microfabricated chip with a three-dimensional topology consisting of lowlands and isolated square highlands (Tepuis), which stand hundreds of microns above the lowlands, in order to assess cancer cell metastatic potential as they invade the highlands. As a test case, the invasive ascents of the Tepui by highly metastatic PC-3 and noninvasive LNCaP prostate cancer cells were used. The vertical ascent by prostate cancer cells from the lowlands to the tops of the Tepui was imaged using confocal microscopy and used as a measure of the relative invasiveness. The less-metastatic cells (LNCaP) never populated all available tops, leaving about 15% of them unoccupied, whereas the more metastatic PC-3 cells occupied all available Tepuis. We argue that this distinct difference in invasiveness is due to contact inhibition.
Original languageEnglish
JournalNational Academy of Sciences. Proceedings
StatePublished - 2011
CitationsWeb of Science® Times Cited: 18
Download as:
Download as PDF
Select render style:
Download as HTML
Select render style:
Download as Word
Select render style:

ID: 6307904