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  • Author: Wernike, Kerstin , Germany

    Institute of Diagnostic Virology, Friedrich-Loeffler- Institut, Germany

  • Author: Bonilauri, Paolo, Italy

    IZSLER, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Italy

  • Author: Dauber, Malte, Germany

    Department of Experimental Animal Facilities and Biorisk Management, Friedrich- Loeffler-Institut, Germany

  • Author: Errington, Jane, United Kingdom

    Animal Health and Veterinary Laboratories Agency, United Kingdom

  • Author: LeBlanc, Neil , Sweden

    Swedish National Veterinary Institute, Virologisk molekylärdiagnostik, Sweden

  • Author: Revilla-Fernández, Sandra, Austria

    Austrian Agency for Health and Food Safety GmbH, Austria

  • Author: Hjulsager, Charlotte Kristiane

    Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Isaksson, Mats, Sweden

    Swedish National Veterinary Institute, Virologisk molekylärdiagnostik, Sweden

  • Author: Stadejek, Tomasz, Poland

    National Veterinary Research Institute, Poland

  • Author: Beer, Martin, Germany

    Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Germany

  • Author: Hoffmann, Bernd , Germany

    Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Germany

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To compare the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays used for the diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV), a Europe-wide interlaboratory ring trial was conducted. A variety of PRRSV strains including North American (NA) and European (EU) genotype isolates were analyzed by the participants. Great differences regarding qualitative diagnostics as well as analytical sensitivity were observed between the individual RT-qPCR systems, especially when investigating strains from the EU genotype. None of the assays or commercial kits used in the ring trial could identify all different PRRSV strains with an optimal analytical and diagnostic sensitivity. The genetic variability of the PRRSV strains, which is supposed to hinder the diagnostic of the RT-PCR because of mutations at the primer binding sites, was also confirmed by sequencing and subsequent phylogenetic analysis. In summary, a major problem in PRRSV diagnostics by RT-qPCR is false-negative results. To achieve maximum safety in the molecular
diagnosis of PRRSV, the combined usage of different assays or kits is highly recommended.
Original languageEnglish
JournalJournal of Veterinary Diagnostic Investigation
Publication date2012
Volume24
Journal number5
Pages855–866
ISSN1040-6387
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 5

Keywords

  • Polymerase chain reaction, Porcine reproductive and respiratory syndrome virus
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