Documents

DOI

  • Author: Wernike, Kerstin

    Institute of Diagnostic Virology, Friedrich-Loeffler- Institut, Germany

  • Author: Bonilauri, Paolo

    IZSLER, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Italy

  • Author: Dauber, Malte

    Department of Experimental Animal Facilities and Biorisk Management, Friedrich- Loeffler-Institut, Germany

  • Author: Errington, Jane

    Animal Health and Veterinary Laboratories Agency, United Kingdom

  • Author: LeBlanc, Neil

    National Veterinary Institute, Sweden

  • Author: Revilla-Fernández, Sandra

    Austrian Agency for Health and Food Safety GmbH, Austria

  • Author: Hjulsager, Charlotte Kristiane

    Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Isaksson, Mats

    National Veterinary Institute, Sweden

  • Author: Stadejek, Tomasz

    National Veterinary Research Institute, Poland

  • Author: Beer, Martin

    Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Germany

  • Author: Hoffmann, Bernd

    Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Germany

View graph of relations

To compare the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays used for the diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV), a Europe-wide interlaboratory ring trial was conducted. A variety of PRRSV strains including North American (NA) and European (EU) genotype isolates were analyzed by the participants. Great differences regarding qualitative diagnostics as well as analytical sensitivity were observed between the individual RT-qPCR systems, especially when investigating strains from the EU genotype. None of the assays or commercial kits used in the ring trial could identify all different PRRSV strains with an optimal analytical and diagnostic sensitivity. The genetic variability of the PRRSV strains, which is supposed to hinder the diagnostic of the RT-PCR because of mutations at the primer binding sites, was also confirmed by sequencing and subsequent phylogenetic analysis. In summary, a major problem in PRRSV diagnostics by RT-qPCR is false-negative results. To achieve maximum safety in the molecular
diagnosis of PRRSV, the combined usage of different assays or kits is highly recommended.
Original languageEnglish
JournalJournal of Veterinary Diagnostic Investigation
Publication date2012
Volume24
Issue5
Pages855–866
ISSN1040-6387
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 8

Keywords

  • Polymerase chain reaction, Porcine reproductive and respiratory syndrome virus
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

Download statistics

No data available

ID: 10146146