Polymer based biosensor for rapid electrochemical detection of virus infection of human cells

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy.Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.
Original languageEnglish
JournalBiosensors and Bioelectronics
Publication date2011
Volume28
Journal number1
Pages386-392
ISSN0956-5663
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 8

Keywords

  • Human cytomegalovirus (CMV), Conductive polymer, Electrochemical impedance spectroscopy (EIS), Atomic force microscopy (AFM), Human fibroblast cells, PEDOT:TsO, Scanning ion conductance microscopy (SICM)
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