Physiological characterisation of Yarrowia lipolytica yields new features of the strain

Publication: Research - peer-reviewConference abstract in journal – Annual report year: 2012



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Yarrowia lipolytica is a potential candidate for the production of value-added compounds from glycerol. Products include citric acid as well as those of the group known as polyols with significant
industrial relevance. In this study the yeast is taken back to a more thorough physiological characterisation in submerged cultures, providing the basis for the formulation of an initial model for the glycerol and glucose metabolism.The strain has been cultivated on glycerol and glucose and a combination of both, this approach has shown that the strain, unlike S. cerevisae, favors glycerol as a substrate over glucose when both are present in a liquid culture. Glycerol is initially utilised via an oxygen dependent pathway, and latter by an oxygen independent pathway, while glucose is utilised latter by aerobic metabolism. Even though the two substrates are metabolised differently, and each of the phases can be clearly distinguished in submerged cultivation, no diauxic growth curve is observed. The maximal specific growth rate on dual substrates is significantly higher than the growth rate on glucose, leading to the hypothesis that the availability of glycerol is boosting the metabolism of glucose. Data also suggest multiple entries to pathways responsible for polyol production as these are produced both during the metabolism of dual substrates and during the metabolism solely of glycerol. Based on analysis of off-gas data and secreted products, metabolic pathways are suggested for the production polyols.
Original languageEnglish
JournalNew Biotechnology
Pages (from-to)S212
StatePublished - 2012
Event15th European Congress on Biotechnology - Istanbul, Turkey


Conference15th European Congress on Biotechnology
LocationThe Grand Cevahir Hotel & Convention Center
CitationsWeb of Science® Times Cited: 0
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ID: 12073464