Physicochemical characterization of fish protein adlayers with bacteria repelling properties

Publication: Research - peer-reviewJournal article – Annual report year: 2013

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Physicochemical characterization of fish protein adlayers with bacteria repelling properties. / Meyer, R. L.; Arpanaei, A.; Pillai, S.; Bernbom, Nete; Enghild, J. J.; Ng, Yin; Gram, Lone; Besenbacher, F.; Kingshott, P.

In: Colloids and Surfaces B: Biointerfaces, Vol. 102, No. 1, 2013, p. 504-510.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

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Meyer, R. L.; Arpanaei, A.; Pillai, S.; Bernbom, Nete; Enghild, J. J.; Ng, Yin; Gram, Lone; Besenbacher, F.; Kingshott, P. / Physicochemical characterization of fish protein adlayers with bacteria repelling properties.

In: Colloids and Surfaces B: Biointerfaces, Vol. 102, No. 1, 2013, p. 504-510.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

Bibtex

@article{a01acfa2b3214484ae7ac86a7c14e6c2,
title = "Physicochemical characterization of fish protein adlayers with bacteria repelling properties",
publisher = "Elsevier BV",
author = "Meyer, {R. L.} and A. Arpanaei and S. Pillai and Nete Bernbom and Enghild, {J. J.} and Yin Ng and Lone Gram and F. Besenbacher and P. Kingshott",
year = "2013",
doi = "10.1016/j.colsurfb.2012.08.044",
volume = "102",
number = "1",
pages = "504--510",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",

}

RIS

TY - JOUR

T1 - Physicochemical characterization of fish protein adlayers with bacteria repelling properties

A1 - Meyer,R. L.

A1 - Arpanaei,A.

A1 - Pillai,S.

A1 - Bernbom,Nete

A1 - Enghild,J. J.

A1 - Ng,Yin

A1 - Gram,Lone

A1 - Besenbacher,F.

A1 - Kingshott,P.

AU - Meyer,R. L.

AU - Arpanaei,A.

AU - Pillai,S.

AU - Bernbom,Nete

AU - Enghild,J. J.

AU - Ng,Yin

AU - Gram,Lone

AU - Besenbacher,F.

AU - Kingshott,P.

PB - Elsevier BV

PY - 2013

Y1 - 2013

N2 - Materials coated with aqueous fish protein extracts can reduce bacterial adhesion, but the mechanism behind the observed effect is not fully understood. In this study we explore the physicochemical properties of fish muscle protein adlayers on four substrates: gold, stainless steel, polystyrene and silicon dioxide. The aims were (i) to determine if the anti-adhesive effect is independent of the underlying substrate chemistry, (ii) to link the physicochemical properties of the adlayer to its ability to repel bacteria, and (iii) to elucidate the mechanism behind this effect. The main proteins on all surfaces were the muscle proteins troponin, tropomyosin, and myosin, and the lipid binding protein apolipoprotein. The quantity, viscoelasticity, and hydration of the protein adlayers varied greatly on the different substrates, but this variation did not affect the bacterial repelling properties. Our results imply that these proteins adsorb to all substrates and provide a steric barrier towards bacterial adhesion, potentially providing a universal antifouling solution.

AB - Materials coated with aqueous fish protein extracts can reduce bacterial adhesion, but the mechanism behind the observed effect is not fully understood. In this study we explore the physicochemical properties of fish muscle protein adlayers on four substrates: gold, stainless steel, polystyrene and silicon dioxide. The aims were (i) to determine if the anti-adhesive effect is independent of the underlying substrate chemistry, (ii) to link the physicochemical properties of the adlayer to its ability to repel bacteria, and (iii) to elucidate the mechanism behind this effect. The main proteins on all surfaces were the muscle proteins troponin, tropomyosin, and myosin, and the lipid binding protein apolipoprotein. The quantity, viscoelasticity, and hydration of the protein adlayers varied greatly on the different substrates, but this variation did not affect the bacterial repelling properties. Our results imply that these proteins adsorb to all substrates and provide a steric barrier towards bacterial adhesion, potentially providing a universal antifouling solution.

KW - Bacterial adhesion

KW - Antifouling

KW - Protein adsorption

KW - MALDI

KW - XPS

KW - QCM-D

U2 - 10.1016/j.colsurfb.2012.08.044

DO - 10.1016/j.colsurfb.2012.08.044

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 1

VL - 102

SP - 504

EP - 510

ER -