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@article{11fb313b7bb447bb9c71b7c2bef5a288,
title = "Optimization of an immunoassay of 2,6-dichlorobenzamide (BAM) and development of regenerative surfaces by immunosorbent modification with newly synthesised BAM hapten library",
publisher = "Elsevier BV",
author = "Basil Uthuppu and Jens Aamand and Claus Jørgensen and Kiersgaard, {Spire M} and Natalie Kostesha and Jakobsen, {Mogens Havsteen}",
year = "2012",
volume = "748",
pages = "95--103",
journal = "Analytica Chimica Acta",
issn = "0003-2670",

}

RIS

TY - JOUR

T1 - Optimization of an immunoassay of 2,6-dichlorobenzamide (BAM) and development of regenerative surfaces by immunosorbent modification with newly synthesised BAM hapten library

A1 - Uthuppu,Basil

A1 - Aamand,Jens

A1 - Jørgensen,Claus

A1 - Kiersgaard,Spire M

A1 - Kostesha,Natalie

A1 - Jakobsen,Mogens Havsteen

AU - Uthuppu,Basil

AU - Aamand,Jens

AU - Jørgensen,Claus

AU - Kiersgaard,Spire M

AU - Kostesha,Natalie

AU - Jakobsen,Mogens Havsteen

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Dichlobenil is an extensively used herbicide worldwide which is transformed to the mobile 2,6-dichlorobenzamide (BAM) in soil. BAM has been found in many European groundwater resources that are exploited for drinking water. Currently, immunoassay based monitoring technique (plate based ELISA) is being employed to quantitatively detect BAM in water samples. In this work, as a starting step of developing immunoassay based on-site monitoring systems for pesticide analysis, the heterogeneous BAM immunoassay is optimised in terms of surface (polymer) regeneration. We have synthesised a small library of BAM haptens which are slightly different in chemical structures, immobilised them on surfaces and compared the affinity constants of the monoclonal antibody HYB 273 towards them. By using ELISA technology, we also have checked the regeneration potentials of the haptens, correlated these results to the affinity constants and found that BAM hapten with an intermediate affinity has better regeneration potential.

AB - Dichlobenil is an extensively used herbicide worldwide which is transformed to the mobile 2,6-dichlorobenzamide (BAM) in soil. BAM has been found in many European groundwater resources that are exploited for drinking water. Currently, immunoassay based monitoring technique (plate based ELISA) is being employed to quantitatively detect BAM in water samples. In this work, as a starting step of developing immunoassay based on-site monitoring systems for pesticide analysis, the heterogeneous BAM immunoassay is optimised in terms of surface (polymer) regeneration. We have synthesised a small library of BAM haptens which are slightly different in chemical structures, immobilised them on surfaces and compared the affinity constants of the monoclonal antibody HYB 273 towards them. By using ELISA technology, we also have checked the regeneration potentials of the haptens, correlated these results to the affinity constants and found that BAM hapten with an intermediate affinity has better regeneration potential.

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

VL - 748

SP - 95

EP - 103

ER -