Non-targeted analysis of tea by hydrophilic interaction liquid chromatography and high resolution mass spectrometry

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Without internal affiliation

  • Author: Fraser, Karl

    AgResearch, New Zealand

  • Author: Harrison, Scott James


  • Author: Lane, Geoff A.

    AgResearch, New Zealand

  • Author: Otter, Don E.

    AgResearch, New Zealand

  • Author: Hemar, Yacine

    University of Auckland, New Zealand

  • Author: Quek, Siew-Young

    University of Auckland, New Zealand

  • Author: Rasmussen, Susanne

    AgResearch, New Zealand

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Tea is the second most consumed beverage in the world and its consumption has been associated with numerous potential health benefits. Factors such as fermentation methods, geographical origin and season can affect the primary and secondary metabolite composition of tea. In this study, a hydrophilic interaction liquid chromatography (HILIC) method coupled to high resolution mass spectrometry in both positive and negative ionisation modes was developed and optimised. The method when combined with principal component analysis to analyse three different types of tea, successfully distinguished samples into different categories, and provided evidence of the metabolites which differed between them. The accurate mass and high resolution attributes of the mass spectrometric data were utilised and relative quantification data were extracted post-data acquisition on 18 amino acids, showing significant differences in amino acid concentrations between tea types and countries. This study highlights the potential of HILIC chromatography combined with non-targeted mass spectrometric methods to provide a comprehensive understanding of polar metabolites in plant extracts.
Original languageEnglish
JournalFood Chemistry
Issue number3
Pages (from-to)1616-1623
StatePublished - 2012
Externally publishedYes
CitationsWeb of Science® Times Cited: 22
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ID: 35964800