New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria

Publication: Research - peer-reviewJournal article – Annual report year: 1998

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Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria ia is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.
Original languageEnglish
JournalApplied and Environmental Microbiology
Publication date1998
Volume64
Journal number6
Pages2240-2246
ISSN0099-2240
StatePublished
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