TY - ABST

T1 - New producers and new 11-, 19-, and 20-residue peptaibiotics: suzukacillins B and C

A1 - Roehrich,C. R.

A1 - Iversen,Anita

A1 - Degenkolb,T.

A1 - Jaklitsch,W. M.

A1 - Voglmayr,H.

A1 - Thrane,Ulf

A1 - Vilcinskas,A.

A1 - Nielsen,Kristian Fog

A1 - Brueckner,H.

AU - Roehrich,C. R.

AU - Iversen,Anita

AU - Degenkolb,T.

AU - Jaklitsch,W. M.

AU - Voglmayr,H.

AU - Thrane,Ulf

AU - Vilcinskas,A.

AU - Nielsen,Kristian Fog

AU - Brueckner,H.

PB - John/Wiley & Sons Ltd.

PY - 2012

Y1 - 2012

N2 - In the course of a project aimed to assess the significance of antibiotics for the producing organism(s) in the natural habitat, we screened a specimen of the fungicolous fungus Hypocrea phellinicola growing on its natural host Phellinus ferruginosus1. Using a peptaibiomics approach2,3, we detected 19- and 20-residue peptide sequences by (U)HPLC/HR-ESI-QqTOF-MS. Structures of peptaibiotics<br/>found were independently confirmed by analyzing the peptaibiome of an agar plate culture of H. phellinicola CBS 119283 (ex-type) grown under laboratory conditions. Notably, H. phellinicola could be identified as a potent producer of 18-, 19-, (culture) and 20-residue (specimen) peptaibiotics of the suzukacillin-type4. Minor components of the 19-residue peptaibols, herein named suzukacillins C, are assumed to carry a C-terminal residue tentatively assigned as tyrosinol (Tyrol). In addition, the previously isolated suzukacillin B4 was sequenced and shown to be a microheterogeneous mixture of 11-residue peptaibols.

AB - In the course of a project aimed to assess the significance of antibiotics for the producing organism(s) in the natural habitat, we screened a specimen of the fungicolous fungus Hypocrea phellinicola growing on its natural host Phellinus ferruginosus1. Using a peptaibiomics approach2,3, we detected 19- and 20-residue peptide sequences by (U)HPLC/HR-ESI-QqTOF-MS. Structures of peptaibiotics<br/>found were independently confirmed by analyzing the peptaibiome of an agar plate culture of H. phellinicola CBS 119283 (ex-type) grown under laboratory conditions. Notably, H. phellinicola could be identified as a potent producer of 18-, 19-, (culture) and 20-residue (specimen) peptaibiotics of the suzukacillin-type4. Minor components of the 19-residue peptaibols, herein named suzukacillins C, are assumed to carry a C-terminal residue tentatively assigned as tyrosinol (Tyrol). In addition, the previously isolated suzukacillin B4 was sequenced and shown to be a microheterogeneous mixture of 11-residue peptaibols.

KW - BIOCHEMISTRY

KW - CHEMISTRY,

JO - Journal of Peptide Science

JF - Journal of Peptide Science

SN - 1075-2617

IS - S1

VL - 18

SP - S73-S73

ER -