Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator. / Cagliani, Alberto; Kosaka, Priscila; Tamayo, Javier; Davis, Zachary James.

In: Lab On a Chip, Vol. 12, No. 11, 2012, p. 2069-2073.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Author

Cagliani, Alberto; Kosaka, Priscila; Tamayo, Javier; Davis, Zachary James / Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator.

In: Lab On a Chip, Vol. 12, No. 11, 2012, p. 2069-2073.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{fb3285e5eea141309f06667692d14052,
title = "Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator",
publisher = "Royal Society of Chemistry",
author = "Alberto Cagliani and Priscila Kosaka and Javier Tamayo and Davis, {Zachary James}",
year = "2012",
doi = "10.1039/c2lc40047b",
volume = "12",
number = "11",
pages = "2069--2073",
journal = "Lab On a Chip",
issn = "1473-0197",

}

RIS

TY - JOUR

T1 - Monitoring the hydration of DNA self-assembled monolayers using an extensional nanomechanical resonator

A1 - Cagliani,Alberto

A1 - Kosaka,Priscila

A1 - Tamayo,Javier

A1 - Davis,Zachary James

AU - Cagliani,Alberto

AU - Kosaka,Priscila

AU - Tamayo,Javier

AU - Davis,Zachary James

PB - Royal Society of Chemistry

PY - 2012

Y1 - 2012

N2 - We have fabricated an ultrasensitive nanomechanical resonator based on the extensional vibration mode to weigh the adsorbed water on self-assembled monolayers of DNA as a function of the relative humidity. The water adsorption isotherms provide the number of adsorbed water molecules per nucleotide for monolayers of single stranded (ss) DNA and after hybridization with the complementary DNA strand. Our results differ from previous data obtained with bulk samples, showing the genuine behavior of these self-assembled monolayers. The hybridization cannot be inferred from the water adsorption isotherms due to the low hybridization efficiency of these highly packed monolayers. Strikingly, we efficiently detect the hybridization by measuring the thermal desorption of water at constant relativity humidity. This finding adds a new nanomechanical tool for developing a label-free nucleic acid sensor based on the interaction between water and self-assembled monolayers of nucleic acids.

AB - We have fabricated an ultrasensitive nanomechanical resonator based on the extensional vibration mode to weigh the adsorbed water on self-assembled monolayers of DNA as a function of the relative humidity. The water adsorption isotherms provide the number of adsorbed water molecules per nucleotide for monolayers of single stranded (ss) DNA and after hybridization with the complementary DNA strand. Our results differ from previous data obtained with bulk samples, showing the genuine behavior of these self-assembled monolayers. The hybridization cannot be inferred from the water adsorption isotherms due to the low hybridization efficiency of these highly packed monolayers. Strikingly, we efficiently detect the hybridization by measuring the thermal desorption of water at constant relativity humidity. This finding adds a new nanomechanical tool for developing a label-free nucleic acid sensor based on the interaction between water and self-assembled monolayers of nucleic acids.

U2 - 10.1039/c2lc40047b

DO - 10.1039/c2lc40047b

JO - Lab On a Chip

JF - Lab On a Chip

SN - 1473-0197

IS - 11

VL - 12

SP - 2069

EP - 2073

ER -