• Author: Cao, Xiaoli

    Department of General Surgery, Peking University First Hospital, Beijing 100034, China. WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, National Food Institute, Technical University of Denmark, Lyngby, Denmark. Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, China

  • Author: Cavaco, Lina

    Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Bülowsvej 27, 1790, København V, Denmark

  • Author: Lv, Yuan

    Peking University First Hospital

  • Author: Li, Yun

    Peking University First Hospital

  • Author: Zheng, Bo

    Peking University First Hospital

  • Author: Wang, Pengyuan

    Department of General Surgery, Peking University First Hospital, Beijing 100034, China. WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, National Food Institute, Technical University of Denmark, Lyngby, Denmark. Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, China

  • Author: Hasman, Henrik

    Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Liu, Yucun

    Department of General Surgery, Peking University First Hospital, Beijing 100034, China. WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, National Food Institute, Technical University of Denmark, Lyngby, Denmark. Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, China

  • Author: Aarestrup, Frank Møller

    Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

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A total of 222 urinary Escherichia coli isolates from 20 tertiary hospitals in 15 different provinces and 4 municipalities in mainland China were characterized by antimicrobial susceptibility, phylogrouping, and the presence of plasmid-mediated quinolone resistance genes. A subset of 138 suspected extended-spectrum cephalosporinase (ESC) producers were examined for genes encoding cephalosporin resistance. Forty-three isolates harboring blaCTX-M-14 or blaCTX-M-15 were analyzed by pulsed-field gel electrophoresis (PFGE), and plasmids containing these genes were typed using PCR-based replicon typing (PBRT). Thirteen phylogroup B2 blaCTX-M-14- and blaCTX-M-15-positive isolates were analyzed by multilocus sequence typing (MLST). A frequent occurrence of resistance (>46%) was observed toward cephalosporins, gentamicin, and fluoroquinolones. Among the 222 isolates, 4 qnrS1, 4 qepA, and 16 aac(6')-Ib-cr genes were confirmed. Four major phylogroups (A, B1, B2, and D) and nontypeable isolates (NTs) were found among the isolates, with phylogroup D (54%) being the most common phylogroup. A total of 110 (80%) of the 138 screened isolates harbored blaCTX-M genes, with blaCTX-M-14 (71%) and blaCTX-M-15 (24%) being the most prevalent of these genes. Nine of the 13 CTX-M-15- or CTX-M-14-containing B2 isolates belonged to ST131. PFGE typing showed a high level of diversity, and plasmid analysis indicated a very large pool of different resistance plasmids mediating the spread of blaCTX-M genes in mainland China. An equally very high frequency of resistance and equally high levels of diversity in phylogroups, PFGE types, and plasmids were observed among community- and hospital-acquired E. coli isolates, indicating the presence of a large reservoir in the community and a long-term spread of cephalosporin resistance in China.
Original languageEnglish
JournalJournal of Clinical Microbiology
Publication date2011
Volume49
Journal number7
Pages2496-2501
ISSN0095-1137
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 8
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