Without internal affiliation

  • Author: Miro-Julia, C.

    Centre Esther Koplowitz, Fundació Clínic per a la Recerca Biomèdica, Spain

  • Author: Rosello, S.

    Servei d’Immunologia and Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

  • Author: Martinez, V.G.

    Centre Esther Koplowitz, Fundació Clínic per a la Recerca Biomèdica, Spain

  • Author: Fink, Dorte Rosenbek

    Unknown

  • Author: Escoda-Ferran, C.

    Centre Esther Koplowitz, Fundació Clínic per a la Recerca Biomèdica, Spain

  • Author: Padilla, O.

    Servei d’Immunologia and Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

  • Author: Vazquez-Echeverria, C.

    Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Spain

  • Author: Espinal-Marin, P.

    Servei di Microbiologia, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

  • Author: Pujades, C.

    Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Spain

  • Author: Garcia-Pardo, A.

    Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Spain

  • Author: Vila, Jordi

    Servei di Microbiologia, Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

  • Author: Serra-Pagès, Carles

    Servei d’Immunologia and Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

  • Author: Holmskov, Uffe

    University of Southern Denmark, Denmark

  • Author: Yélamos, José

    Servei d'Immunologia, Hospital del Mar, Institut Municipal d'Investigacions Mèdiques, Spain

  • Author: Lozano, Francisco

    Servei d’Immunologia and Hospital Clínic de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Spain

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The scavenger receptor cysteine-rich superfamily (SRCR-SF) members are transmembrane and/or secreted receptors exhibiting one or several repeats of a cysteine-rich protein module of ∼100 aa, named scavenger receptor cysteine-rich (SRCR). Two types of SRCR domains (A or B) have been reported, which differ in the number of coding exons and intradomain cysteines. Although no unifying function has been reported for SRCR-SF members, recognition of pathogen-associated molecular patterns (PAMPs) was recently shown for some of them. In this article, we report the structural and functional characterization of mouse S5D-SRCRB, a new group B member of the SRCR-SF. The s5d-srcrb gene maps at mouse chromosome 7 and encompasses 14 exons extending over 15 kb. The longest cDNA sequence found is 4286 bp in length and encodes a mature protein of 1371 aa, with a predicted Mr of 144.6 kDa. Using an episomal mammalian-expression system, a glycosylated soluble recombinant form >200 kDa was obtained and used as immunogen for the generation of specific rat mAbs. Subsequent immunohistochemical and real-time PCR analysis showed significant S5D-SRCRB expression in murine genitourinary and digestive tracts. S5D-SRCRB was shown to bind endogenous extracellular matrix proteins (laminin and galectin-1), as well as PAMPs present on Gram-positive and Gram-negative bacteria and fungi. PAMP binding by S5D-SRCRB induced microbial aggregation and subsequent inhibition of PAMP-induced cytokine release. These abilities suggest that S5D-SRCRB might play a role in the innate defense and homeostasis of certain specialized epithelial surfaces.
Original languageEnglish
JournalJournal of Immunology
Publication date2011
Volume186
Issue4
Pages2344-2354
ISSN0022-1767
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 6
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