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@article{d57a05f1cd9748a6952519a2f360e0ce,
title = "Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus",
keywords = "Pharyngeal epithelium, Cytokine mRNA, Foot-and-mouth disease",
publisher = "W.B./Saunders Co. Ltd.",
author = "Stenfeldt, {Anna Carolina} and Heegaard, {Peter M. H.} and Anders Stockmarr and Graham Belsham",
year = "2012",
doi = "10.1016/j.jcpa.2011.06.005",
volume = "146",
number = "2-3",
pages = "243--252",
journal = "Journal of Comparative Pathology",
issn = "0021-9975",

}

RIS

TY - JOUR

T1 - Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

A1 - Stenfeldt,Anna Carolina

A1 - Heegaard,Peter M. H.

A1 - Stockmarr,Anders

A1 - Belsham,Graham

AU - Stenfeldt,Anna Carolina

AU - Heegaard,Peter M. H.

AU - Stockmarr,Anders

AU - Belsham,Graham

PB - W.B./Saunders Co. Ltd.

PY - 2012

Y1 - 2012

N2 - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection. There were also significantly higher levels of TNF-a mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.

AB - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection. There were also significantly higher levels of TNF-a mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.

KW - Pharyngeal epithelium

KW - Cytokine mRNA

KW - Foot-and-mouth disease

U2 - 10.1016/j.jcpa.2011.06.005

DO - 10.1016/j.jcpa.2011.06.005

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

SN - 0021-9975

IS - 2-3

VL - 146

SP - 243

EP - 252

ER -