View graph of relations

A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection. There were also significantly higher levels of TNF-a mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.
Original languageEnglish
JournalJournal of Comparative Pathology
Issue number2-3
Pages (from-to)243-252
StatePublished - 2012
CitationsWeb of Science® Times Cited: 10


  • Pharyngeal epithelium, Cytokine mRNA, Foot-and-mouth disease
Download as:
Download as PDF
Select render style:
Download as HTML
Select render style:
Download as Word
Select render style:

ID: 6320979