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Modeling of Phenoxy Acid Herbicide Mineralization and Growth of Microbial Degraders in 15 Soils Monitored by Quantitative Real-Time PCR of the Functional tfdA Gene. / Bælum, Jacob; Prestat, Emmanuel; David, Maude M.; Strobel, Bjarne W.; Jacobsen, Carsten Suhr.

In: Applied and Environmental Microbiology, Vol. 78, No. 15, 2012, p. 5305-5312.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Bælum, Jacob; Prestat, Emmanuel; David, Maude M.; Strobel, Bjarne W.; Jacobsen, Carsten Suhr / Modeling of Phenoxy Acid Herbicide Mineralization and Growth of Microbial Degraders in 15 Soils Monitored by Quantitative Real-Time PCR of the Functional tfdA Gene.

In: Applied and Environmental Microbiology, Vol. 78, No. 15, 2012, p. 5305-5312.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{44fc41a5faf740f39a3c0abe8801d52a,
title = "Modeling of Phenoxy Acid Herbicide Mineralization and Growth of Microbial Degraders in 15 Soils Monitored by Quantitative Real-Time PCR of the Functional tfdA Gene",
publisher = "American Society for Microbiology",
author = "Jacob Bælum and Emmanuel Prestat and David, {Maude M.} and Strobel, {Bjarne W.} and Jacobsen, {Carsten Suhr}",
year = "2012",
doi = "10.1128/AEM.00990-12",
volume = "78",
number = "15",
pages = "5305--5312",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",

}

RIS

TY - JOUR

T1 - Modeling of Phenoxy Acid Herbicide Mineralization and Growth of Microbial Degraders in 15 Soils Monitored by Quantitative Real-Time PCR of the Functional tfdA Gene

A1 - Bælum,Jacob

A1 - Prestat,Emmanuel

A1 - David,Maude M.

A1 - Strobel,Bjarne W.

A1 - Jacobsen,Carsten Suhr

AU - Bælum,Jacob

AU - Prestat,Emmanuel

AU - David,Maude M.

AU - Strobel,Bjarne W.

AU - Jacobsen,Carsten Suhr

PB - American Society for Microbiology

PY - 2012

Y1 - 2012

N2 - Mineralization potentials, rates, and kinetics of the three phenoxy acid (PA) herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 4-chloro-2-methylphenoxyacetic acid (MCPA), and 2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP), were investigated and compared in 15 soils collected from five continents. The mineralization patterns were fitted by zero/linear or exponential growth forms of the three-half-order models and by logarithmic (log), first-order, or zero-order kinetic models. Prior and subsequent to the mineralization event, tfdA genes were quantified using real-time PCR to estimate the genetic potential for degrading PA in the soils. In 25 of the 45 mineralization scenarios, ∼60% mineralization was observed within 118 days. Elevated concentrations of tfdA in the range 1 × 105 to 5 × 107 gene copies g−1 of soil were observed in soils where mineralization could be described by using growth-linked kinetic models. A clear trend was observed that the mineralization rates of the three PAs occurred in the order 2,4-D > MCPA > MCPP, and a correlation was observed between rapid mineralization and soils exposed to PA previously. Finally, for 2,4-D mineralization, all seven mineralization patterns which were best fitted by the exponential model yielded a higher tfdA gene potential after mineralization had occurred than the three mineralization patterns best fitted by the Lin model.

AB - Mineralization potentials, rates, and kinetics of the three phenoxy acid (PA) herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 4-chloro-2-methylphenoxyacetic acid (MCPA), and 2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP), were investigated and compared in 15 soils collected from five continents. The mineralization patterns were fitted by zero/linear or exponential growth forms of the three-half-order models and by logarithmic (log), first-order, or zero-order kinetic models. Prior and subsequent to the mineralization event, tfdA genes were quantified using real-time PCR to estimate the genetic potential for degrading PA in the soils. In 25 of the 45 mineralization scenarios, ∼60% mineralization was observed within 118 days. Elevated concentrations of tfdA in the range 1 × 105 to 5 × 107 gene copies g−1 of soil were observed in soils where mineralization could be described by using growth-linked kinetic models. A clear trend was observed that the mineralization rates of the three PAs occurred in the order 2,4-D > MCPA > MCPP, and a correlation was observed between rapid mineralization and soils exposed to PA previously. Finally, for 2,4-D mineralization, all seven mineralization patterns which were best fitted by the exponential model yielded a higher tfdA gene potential after mineralization had occurred than the three mineralization patterns best fitted by the Lin model.

U2 - 10.1128/AEM.00990-12

DO - 10.1128/AEM.00990-12

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 15

VL - 78

SP - 5305

EP - 5312

ER -