Mobilization of "non-mobilizable" plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis

Publication: Research - peer-reviewJournal article – Annual report year: 1996

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Mobilization of "non-mobilizable" plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis. / Andrup, Lars; Jørgensen, Ole; Wilcks, Andrea; Ørum-Smidt, Lasse; Jensen, Gert Bolander.

In: Plasmid, Vol. 36, No. 2, 1996, p. 75-85.

Publication: Research - peer-reviewJournal article – Annual report year: 1996

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Andrup, Lars; Jørgensen, Ole; Wilcks, Andrea; Ørum-Smidt, Lasse; Jensen, Gert Bolander / Mobilization of "non-mobilizable" plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis.

In: Plasmid, Vol. 36, No. 2, 1996, p. 75-85.

Publication: Research - peer-reviewJournal article – Annual report year: 1996

Bibtex

@article{f2da37f4e6664cfda5f97ba558b77559,
title = "Mobilization of {"}non-mobilizable{"} plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis",
publisher = "Academic Press",
author = "Lars Andrup and Ole Jørgensen and Andrea Wilcks and Lasse Ørum-Smidt and Jensen, {Gert Bolander}",
year = "1996",
doi = "10.1006/plas.1996.0035",
volume = "36",
number = "2",
pages = "75--85",
journal = "Plasmid",
issn = "0147-619X",

}

RIS

TY - JOUR

T1 - Mobilization of "non-mobilizable" plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis

A1 - Andrup,Lars

A1 - Jørgensen,Ole

A1 - Wilcks,Andrea

A1 - Ørum-Smidt,Lasse

A1 - Jensen,Gert Bolander

AU - Andrup,Lars

AU - Jørgensen,Ole

AU - Wilcks,Andrea

AU - Ørum-Smidt,Lasse

AU - Jensen,Gert Bolander

PB - Academic Press

PY - 1996

Y1 - 1996

N2 - The aggregation-mediated conjugation system of Bacillus thuringiensis subsp. israelensis (Bti), encoded by the 200-kb plasmid pXO16, is highly potent in transferring itself and efficient in mobilizing other nonconjugative plasmids. In the present study we have analyzed the native Bacillus cereus plasmid pBC16. This plasmid has previously been shown to harbor a mob gene (ORF beta) and a locus functioning as an oriT site in plasmid pLS20-mediated conjugation in Bacillus subtilis. However, in the conjugation system of Bti we found that a derivative of pBC16 deleted for both these loci was mobilizable, although at a reduced frequency. Another derivative of pBC16, containing a deletion spanning the Brst half of the coding region of the mob gene, was found to be nearly as mobilizable as the intact pBC16, suggesting its dispensability in the transfer process. Other plasmids based on the theta-replicating origins, pAM beta 1, pLS20, ori43, ori44, and ori60, were also consistently mobilized in the conjugation system encoded by Bti plasmid pXO16. Analyzing the conjugation process by the use of scanning electron microscopy revealed the presence of connections between cells in the mating mixtures. These connections did not appear in monocultures of the donor strain or the recipient strain and may be conjugational junctions. (C) 1996 Academic Press, Inc.

AB - The aggregation-mediated conjugation system of Bacillus thuringiensis subsp. israelensis (Bti), encoded by the 200-kb plasmid pXO16, is highly potent in transferring itself and efficient in mobilizing other nonconjugative plasmids. In the present study we have analyzed the native Bacillus cereus plasmid pBC16. This plasmid has previously been shown to harbor a mob gene (ORF beta) and a locus functioning as an oriT site in plasmid pLS20-mediated conjugation in Bacillus subtilis. However, in the conjugation system of Bti we found that a derivative of pBC16 deleted for both these loci was mobilizable, although at a reduced frequency. Another derivative of pBC16, containing a deletion spanning the Brst half of the coding region of the mob gene, was found to be nearly as mobilizable as the intact pBC16, suggesting its dispensability in the transfer process. Other plasmids based on the theta-replicating origins, pAM beta 1, pLS20, ori43, ori44, and ori60, were also consistently mobilized in the conjugation system encoded by Bti plasmid pXO16. Analyzing the conjugation process by the use of scanning electron microscopy revealed the presence of connections between cells in the mating mixtures. These connections did not appear in monocultures of the donor strain or the recipient strain and may be conjugational junctions. (C) 1996 Academic Press, Inc.

U2 - 10.1006/plas.1996.0035

DO - 10.1006/plas.1996.0035

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 2

VL - 36

SP - 75

EP - 85

ER -