Microfluidic device to study cell transmigration under physiological shear stress conditions

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Standard

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{bb849ab8419e4d63ae8b9121aeebc4f2,
title = "Microfluidic device to study cell transmigration under physiological shear stress conditions",
author = "Dorota Kwasny and Katrine Kiilerich-Pedersen and Moresco, {Jacob Lange} and Maria Dimaki and Noemi Rozlosnik and Svendsen, {Winnie Edith}",
year = "2011",
doi = "10.1007/s10544-011-9559-x",
volume = "13",
number = "5",
pages = "899--907",
journal = "Biomedical Microdevices",
issn = "13872176",

}

RIS

TY - JOUR

T1 - Microfluidic device to study cell transmigration under physiological shear stress conditions

A1 - Kwasny,Dorota

A1 - Kiilerich-Pedersen,Katrine

A1 - Moresco,Jacob Lange

A1 - Dimaki,Maria

A1 - Rozlosnik,Noemi

A1 - Svendsen,Winnie Edith

AU - Kwasny,Dorota

AU - Kiilerich-Pedersen,Katrine

AU - Moresco,Jacob Lange

AU - Dimaki,Maria

AU - Rozlosnik,Noemi

AU - Svendsen,Winnie Edith

PY - 2011

Y1 - 2011

N2 - The development of new drug therapies relies on studies of cell transmigration in in vitro systems. Migration has traditionally been studied using two methods, the Boyden chamber and a shear flow chamber assay. Though, commonly applied in cell transmigration studies, they are far from imitating a natural migration process. Here we describe a novel in vitro cell transmigration microfluidic assay, which mimicks physiological shear flow conditions in blood vessels. The device was designed to incorporate the principles of both the Boyden chamber and the shear flow chamber assay, i.e. migration through the membrane under flow conditions. The 3D environment of migrating cells is imitated by injecting cell adhesion proteins to coat the membrane in the device. We tested the developed device with Jurkat cells migration towards medium supplemented with serum, and with chemokine induced lymphocytes migration. The applied continuous flow of cell suspension and chemoattractant ensures that the concentration gradient is maintained in time and space. The cell adhesion proteins used to enhance cell migration in the device were fibronectin and VCAM-1. We successfully observed a multistep transmigration process by means of the developed microfluidic migration assay. The presented device is inexpensive, easy to fabricate and disposable, having a potential to be applied in basic research as well as in the drug development process.

AB - The development of new drug therapies relies on studies of cell transmigration in in vitro systems. Migration has traditionally been studied using two methods, the Boyden chamber and a shear flow chamber assay. Though, commonly applied in cell transmigration studies, they are far from imitating a natural migration process. Here we describe a novel in vitro cell transmigration microfluidic assay, which mimicks physiological shear flow conditions in blood vessels. The device was designed to incorporate the principles of both the Boyden chamber and the shear flow chamber assay, i.e. migration through the membrane under flow conditions. The 3D environment of migrating cells is imitated by injecting cell adhesion proteins to coat the membrane in the device. We tested the developed device with Jurkat cells migration towards medium supplemented with serum, and with chemokine induced lymphocytes migration. The applied continuous flow of cell suspension and chemoattractant ensures that the concentration gradient is maintained in time and space. The cell adhesion proteins used to enhance cell migration in the device were fibronectin and VCAM-1. We successfully observed a multistep transmigration process by means of the developed microfluidic migration assay. The presented device is inexpensive, easy to fabricate and disposable, having a potential to be applied in basic research as well as in the drug development process.

KW - Shear stress

KW - Lymphocytes

KW - Cell migration

KW - Microfluidic migration assay

U2 - 10.1007/s10544-011-9559-x

DO - 10.1007/s10544-011-9559-x

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 13872176

IS - 5

VL - 13

SP - 899

EP - 907

ER -