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@article{528d83dec0774a0b9c606cd376f20281,
title = "Microbial Community-Level Physiological Profiles (CLPP) and herbicide mineralization potential in groundwater affected by agricultural land use",
keywords = "Diversity, Microbial degradation, Pesticide, Aquifer, Biolog EcoPlate",
publisher = "Elsevier BV",
author = "Janniche, {Gry Sander} and Henrik Spliid and Hans-Jørgen Albrechtsen",
year = "2012",
doi = "10.1016/j.jconhyd.2012.08.008",
volume = "140",
pages = "45--55",
journal = "Journal of Contaminant Hydrology",
issn = "0169-7722",

}

RIS

TY - JOUR

T1 - Microbial Community-Level Physiological Profiles (CLPP) and herbicide mineralization potential in groundwater affected by agricultural land use

A1 - Janniche,Gry Sander

A1 - Spliid,Henrik

A1 - Albrechtsen,Hans-Jørgen

AU - Janniche,Gry Sander

AU - Spliid,Henrik

AU - Albrechtsen,Hans-Jørgen

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Diffuse groundwater pollution from agricultural land use may impact the microbial groundwater community, which was investigated as Community-Level Physiological Profiles (CLPP) using EcoPlate™. Water was sampled from seven piezometers and a spring in a small agricultural catchment with diffuse herbicide and nitrate pollution. Based on the Shannon–Wiener and Simpson's diversity indices the diversity in the microbial communities was high. The response from the EcoPlates™ showed which substrates support groundwater bacteria, and all 31 carbon sources were utilized by organisms from at least one water sample. However, only nine carbon sources were utilized by all water samples: d-Mannitol, N-acetyl-d-glucosamine, putrescine, d-galacturonic acid, itaconic acid, 4-hydroxy benzoic acid, tween 40, tween 80, and l-asparagine. In all water samples the microorganisms preferred d-mannitol, d-galacturonic acid, tween 40, and 4-hydroxy benzoic acid as substrates, whereas none preferred 2-hydroxy benzoic acid, α-d-lactose, d,l-α-glycerol phosphate, α-ketobutyric acid, l-threonine and glycyl-l-glutamic acid. Principal Component Analysis of the CLPP's clustered the most agriculturally affected groundwater samples, indicating that the agricultural land use affects the groundwater microbial communities. Furthermore, the ability to mineralize atrazine and isoproturon, which have been used in the catchment, was also associated with this cluster.

AB - Diffuse groundwater pollution from agricultural land use may impact the microbial groundwater community, which was investigated as Community-Level Physiological Profiles (CLPP) using EcoPlate™. Water was sampled from seven piezometers and a spring in a small agricultural catchment with diffuse herbicide and nitrate pollution. Based on the Shannon–Wiener and Simpson's diversity indices the diversity in the microbial communities was high. The response from the EcoPlates™ showed which substrates support groundwater bacteria, and all 31 carbon sources were utilized by organisms from at least one water sample. However, only nine carbon sources were utilized by all water samples: d-Mannitol, N-acetyl-d-glucosamine, putrescine, d-galacturonic acid, itaconic acid, 4-hydroxy benzoic acid, tween 40, tween 80, and l-asparagine. In all water samples the microorganisms preferred d-mannitol, d-galacturonic acid, tween 40, and 4-hydroxy benzoic acid as substrates, whereas none preferred 2-hydroxy benzoic acid, α-d-lactose, d,l-α-glycerol phosphate, α-ketobutyric acid, l-threonine and glycyl-l-glutamic acid. Principal Component Analysis of the CLPP's clustered the most agriculturally affected groundwater samples, indicating that the agricultural land use affects the groundwater microbial communities. Furthermore, the ability to mineralize atrazine and isoproturon, which have been used in the catchment, was also associated with this cluster.

KW - Diversity

KW - Microbial degradation

KW - Pesticide

KW - Aquifer

KW - Biolog EcoPlate

U2 - 10.1016/j.jconhyd.2012.08.008

DO - 10.1016/j.jconhyd.2012.08.008

JO - Journal of Contaminant Hydrology

JF - Journal of Contaminant Hydrology

SN - 0169-7722

VL - 140

SP - 45

EP - 55

ER -