Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

Publication: Research - peer-reviewJournal article – Annual report year: 2011

NullPointerException

View graph of relations

We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.
Original languageEnglish
JournalApplied and Environmental Microbiology
Publication date2011
Volume77
Journal number23
Pages8456-8458
ISSN0099-2240
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 3
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 6237868