Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study. / Pullinger, B; Profka, H; Ardenkjaer-Larsen, J H; Kuzma, N N; Kadlecek, S; Rizi, R R.

In: N M R in Biomedicine, Vol. 25, No. 10, 2012, p. 1113-1118.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Pullinger, B; Profka, H; Ardenkjaer-Larsen, J H; Kuzma, N N; Kadlecek, S; Rizi, R R / Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study.

In: N M R in Biomedicine, Vol. 25, No. 10, 2012, p. 1113-1118.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{0ec430ff3da14a278bd9e80ecff765f8,
title = "Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study",
publisher = "John/Wiley & Sons Ltd.",
author = "B Pullinger and H Profka and Ardenkjaer-Larsen, {J H} and Kuzma, {N N} and S Kadlecek and Rizi, {R R}",
year = "2012",
doi = "10.1002/nbm.2777",
volume = "25",
number = "10",
pages = "1113--1118",
journal = "N M R in Biomedicine",
issn = "0952-3480",

}

RIS

TY - JOUR

T1 - Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

A1 - Pullinger,B

A1 - Profka,H

A1 - Ardenkjaer-Larsen,J H

A1 - Kuzma,N N

A1 - Kadlecek,S

A1 - Rizi,R R

AU - Pullinger,B

AU - Profka,H

AU - Ardenkjaer-Larsen,J H

AU - Kuzma,N N

AU - Kadlecek,S

AU - Rizi,R R

PB - John/Wiley & Sons Ltd.

PY - 2012

Y1 - 2012

N2 - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.

AB - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.

U2 - 10.1002/nbm.2777

DO - 10.1002/nbm.2777

JO - N M R in Biomedicine

JF - N M R in Biomedicine

SN - 0952-3480

IS - 10

VL - 25

SP - 1113

EP - 1118

ER -