Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study
Publication: Research - peer-review › Journal article – Annual report year: 2012
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Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study. / Pullinger, B; Profka, H; Ardenkjaer-Larsen, J H; Kuzma, N N; Kadlecek, S; Rizi, R R.
In: N M R in Biomedicine, Vol. 25, No. 10, 2012, p. 1113-1118.Publication: Research - peer-review › Journal article – Annual report year: 2012
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TY - JOUR
T1 - Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study
A1 - Pullinger,B
A1 - Profka,H
A1 - Ardenkjaer-Larsen,J H
A1 - Kuzma,N N
A1 - Kadlecek,S
A1 - Rizi,R R
AU - Pullinger,B
AU - Profka,H
AU - Ardenkjaer-Larsen,J H
AU - Kuzma,N N
AU - Kadlecek,S
AU - Rizi,R R
PB - John/Wiley & Sons Ltd.
PY - 2012
Y1 - 2012
N2 - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.
AB - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.
U2 - 10.1002/nbm.2777
DO - 10.1002/nbm.2777
JO - N M R in Biomedicine
JF - N M R in Biomedicine
SN - 0952-3480
IS - 10
VL - 25
SP - 1113
EP - 1118
ER -