Media and growth conditions for induction of secondary metabolite production

Publication: Research - peer-reviewJournal article – Annual report year: 2012

View graph of relations

Growth media and incubation conditions have a very strong influence of secondary metabolite production. There is no consensus on which media are the optimal for metabolite production, but a series of useful and effective media and incubation conditions have been listed here. Chemically well-defined media are suited for biochemical studies, but in order to get chemical diversity expressed in filamentous fungi, sources rich in amino acids, vitamins, and trace metals have to be added, such as yeast extract and oatmeal. A battery of solid agar media is recommended for exploration of chemical diversity as agar plug samples are easily analyzed to get an optimal representation of the qualitative secondary metabolome. Standard incubation for a week at 25°C in darkness is recommended, but optimal conditions have to be modified depending on the ecology and physiology of different filamentous fungi.
Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)47-58
StatePublished - 2012


  • Secondary metabolites, Growth media, Agar plugs, Yeast extract, HPLC
Download as:
Download as PDF
Select render style:
Download as HTML
Select render style:
Download as Word
Select render style:

ID: 12685627