Mapping the Complex Morphology of Cell Interactions with Nanowire Substrates Using FIB-SEM

Publication: Research - peer-reviewJournal article – Annual report year: 2013

View graph of relations

Using high resolution focused ion beam scanning electron microscopy (FIB-SEM) we study the details of cell-nanostructure interactions using serial block face imaging. 3T3 Fibroblast cellular monolayers are cultured on flat glass as a control surface and on two types of nanostructured scaffold substrates made from silicon black (Nanograss) with low- and high nanowire density. After culturing for 72 hours the cells were fixed, heavy metal stained, embedded in resin, and processed with FIB-SEM block face imaging without removing the substrate. The sample preparation procedure, image acquisition and image post-processing were specifically optimised for cellular monolayers cultured on nanostructured substrates. Cells display a wide range of interactions with the nanostructures depending on the surface morphology, but also greatly varying from one cell to another on the same substrate, illustrating a wide phenotypic variability. Depending on the substrate and cell, we observe that cells could for instance: break the nanowires and engulf them, flatten the nanowires or simply reside on top of them. Given the complexity of interactions, we have categorised our observations and created an overview map. The results demonstrate that detailed nanoscale resolution images are required to begin understanding the wide variety of individual cells' interactions with a structured substrate. The map will provide a framework for light microscopy studies of such interactions indicating what modes of interactions must be considered.
Original languageEnglish
JournalP L o S One
Volume8
Issue number1
Pages (from-to)e53307
Number of pages12
ISSN1932-6203
DOIs
StatePublished - 2013

Bibliographical note

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This study was supported by FP7-Technotubes, CP-IP 228579-1; FP7-Nanoscale, CP-FP 214566-2 (http://www.nanoscale-fp7.eu/); Technical University of Denmark; Institutes: DTU CEN, DTU Nanotech (www.cen.dtu.dk and www.nanotech.dtu.dk). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

CitationsWeb of Science® Times Cited: 22
Download as:
Download as PDF
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBE/CSEHarvardMLAStandardVancouverShortLong
Word

Download statistics

No data available

ID: 52562463