Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis
Publication: Research - peer-review › Journal article – Annual report year: 2012
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Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. / Johansen, Louise K.; Iburg, Tine M.; Nielsen, Ole L.; Leifsson, Páll S.; Dahl-Petersen, Kirstin; Koch, Janne; Frees, Dorte; Aalbæk, Bent; Heegaard, Peter M.H.; Jensen, Henrik E.
In: Prostaglandins & Other Lipid Mediators, Vol. 97, No. 3-4, 2012, p. 103-108.Publication: Research - peer-review › Journal article – Annual report year: 2012
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TY - JOUR
T1 - Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis
A1 - Johansen,Louise K.
A1 - Iburg,Tine M.
A1 - Nielsen,Ole L.
A1 - Leifsson,Páll S.
A1 - Dahl-Petersen,Kirstin
A1 - Koch,Janne
A1 - Frees,Dorte
A1 - Aalbæk,Bent
A1 - Heegaard,Peter M.H.
A1 - Jensen,Henrik E.
AU - Johansen,Louise K.
AU - Iburg,Tine M.
AU - Nielsen,Ole L.
AU - Leifsson,Páll S.
AU - Dahl-Petersen,Kirstin
AU - Koch,Janne
AU - Frees,Dorte
AU - Aalbæk,Bent
AU - Heegaard,Peter M.H.
AU - Jensen,Henrik E.
PB - Elsevier Inc.
PY - 2012
Y1 - 2012
N2 - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.
AB - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.
KW - Osteomyelitis
KW - Bone morphology
KW - Cyclooxygenase 2
KW - Alkaline phosphatase
KW - Haptoglobin
KW - Porcine models
U2 - 10.1016/j.prostaglandins.2012.01.002
DO - 10.1016/j.prostaglandins.2012.01.002
JO - Prostaglandins & Other Lipid Mediators
JF - Prostaglandins & Other Lipid Mediators
SN - 1098-8823
IS - 3-4
VL - 97
SP - 103
EP - 108
ER -