Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Standard

Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. / Johansen, Louise K.; Iburg, Tine M.; Nielsen, Ole L.; Leifsson, Páll S.; Dahl-Petersen, Kirstin; Koch, Janne; Frees, Dorte; Aalbæk, Bent; Heegaard, Peter M.H.; Jensen, Henrik E.

In: Prostaglandins & Other Lipid Mediators, Vol. 97, No. 3-4, 2012, p. 103-108.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

Johansen, LK, Iburg, TM, Nielsen, OL, Leifsson, PS, Dahl-Petersen, K, Koch, J, Frees, D, Aalbæk, B, Heegaard, PMH & Jensen, HE 2012, 'Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis' Prostaglandins & Other Lipid Mediators, vol 97, no. 3-4, pp. 103-108., 10.1016/j.prostaglandins.2012.01.002

APA

Johansen, L. K., Iburg, T. M., Nielsen, O. L., Leifsson, P. S., Dahl-Petersen, K., Koch, J., Frees, D., Aalbæk, B., Heegaard, P. M. H., & Jensen, H. E. (2012). Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. Prostaglandins & Other Lipid Mediators, 97(3-4), 103-108. 10.1016/j.prostaglandins.2012.01.002

CBE

Johansen LK, Iburg TM, Nielsen OL, Leifsson PS, Dahl-Petersen K, Koch J, Frees D, Aalbæk B, Heegaard PMH, Jensen HE. 2012. Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. Prostaglandins & Other Lipid Mediators. 97(3-4):103-108. Available from: 10.1016/j.prostaglandins.2012.01.002

MLA

Vancouver

Johansen LK, Iburg TM, Nielsen OL, Leifsson PS, Dahl-Petersen K, Koch J et al. Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. Prostaglandins & Other Lipid Mediators. 2012;97(3-4):103-108. Available from: 10.1016/j.prostaglandins.2012.01.002

Author

Johansen, Louise K.; Iburg, Tine M.; Nielsen, Ole L.; Leifsson, Páll S.; Dahl-Petersen, Kirstin; Koch, Janne; Frees, Dorte; Aalbæk, Bent; Heegaard, Peter M.H.; Jensen, Henrik E. / Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis.

In: Prostaglandins & Other Lipid Mediators, Vol. 97, No. 3-4, 2012, p. 103-108.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{6b2db62dbb724657b62ef5cb8e342001,
title = "Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis",
publisher = "Elsevier Inc.",
author = "Johansen, {Louise K.} and Iburg, {Tine M.} and Nielsen, {Ole L.} and Leifsson, {Páll S.} and Kirstin Dahl-Petersen and Janne Koch and Dorte Frees and Bent Aalbæk and Heegaard, {Peter M.H.} and Jensen, {Henrik E.}",
year = "2012",
doi = "10.1016/j.prostaglandins.2012.01.002",
volume = "97",
number = "3-4",
pages = "103--108",
journal = "Prostaglandins & Other Lipid Mediators",
issn = "1098-8823",

}

RIS

TY - JOUR

T1 - Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

A1 - Johansen,Louise K.

A1 - Iburg,Tine M.

A1 - Nielsen,Ole L.

A1 - Leifsson,Páll S.

A1 - Dahl-Petersen,Kirstin

A1 - Koch,Janne

A1 - Frees,Dorte

A1 - Aalbæk,Bent

A1 - Heegaard,Peter M.H.

A1 - Jensen,Henrik E.

AU - Johansen,Louise K.

AU - Iburg,Tine M.

AU - Nielsen,Ole L.

AU - Leifsson,Páll S.

AU - Dahl-Petersen,Kirstin

AU - Koch,Janne

AU - Frees,Dorte

AU - Aalbæk,Bent

AU - Heegaard,Peter M.H.

AU - Jensen,Henrik E.

PB - Elsevier Inc.

PY - 2012

Y1 - 2012

N2 - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.

AB - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.

KW - Osteomyelitis

KW - Bone morphology

KW - Cyclooxygenase 2

KW - Alkaline phosphatase

KW - Haptoglobin

KW - Porcine models

U2 - 10.1016/j.prostaglandins.2012.01.002

DO - 10.1016/j.prostaglandins.2012.01.002

JO - Prostaglandins & Other Lipid Mediators

JF - Prostaglandins & Other Lipid Mediators

SN - 1098-8823

IS - 3-4

VL - 97

SP - 103

EP - 108

ER -