Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Publication: Research - peer-reviewJournal article – Annual report year: 2012

  • Author: Johansen, Louise K.

    University of Copenhagen, Denmark

  • Author: Iburg, Tine M.

    University of Copenhagen, Denmark

  • Author: Nielsen, Ole L.

    University of Copenhagen, Denmark

  • Author: Leifsson, Páll S.

    University of Copenhagen, Denmark

  • Author: Dahl-Petersen, Kirstin

    University of Copenhagen, Denmark

  • Author: Koch, Janne

    University of Copenhagen, Denmark

  • Author: Frees, Dorte

    University of Copenhagen, Denmark

  • Author: Aalbæk, Bent

    University of Copenhagen, Denmark

  • Author: Heegaard, Peter M.H.

    Innate Immunology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Jensen, Henrik E.

    University of Copenhagen, Denmark

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It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.
Original languageEnglish
JournalProstaglandins & Other Lipid Mediators
Publication date2012
Volume97
Issue3-4
Pages103-108
ISSN1098-8823
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 0

Keywords

  • Osteomyelitis, Bone morphology, Cyclooxygenase 2, Alkaline phosphatase, Haptoglobin, Porcine models
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