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Lactococcal Abortive Infection Protein AbiV Interacts Directly with the Phage Protein SaV and Prevents Translation of Phage Proteins. / Haaber, Jakob Brandt Borup; Samson, J.E.; Labrie, S.J.; Campanacci, V.; Cambillau, C.; Moineau, S.; Hammer, Karin.

In: Applied and Environmental Microbiology, Vol. 76, No. 21, 2010, p. 7085-7092.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Haaber, Jakob Brandt Borup; Samson, J.E.; Labrie, S.J.; Campanacci, V.; Cambillau, C.; Moineau, S.; Hammer, Karin / Lactococcal Abortive Infection Protein AbiV Interacts Directly with the Phage Protein SaV and Prevents Translation of Phage Proteins.

In: Applied and Environmental Microbiology, Vol. 76, No. 21, 2010, p. 7085-7092.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{30bfcc7cfa5d4f108487c87052552188,
title = "Lactococcal Abortive Infection Protein AbiV Interacts Directly with the Phage Protein SaV and Prevents Translation of Phage Proteins",
publisher = "American Society for Microbiology",
author = "Haaber, {Jakob Brandt Borup} and J.E. Samson and S.J. Labrie and V. Campanacci and C. Cambillau and S. Moineau and Karin Hammer",
year = "2010",
doi = "10.1128/AEM.00093-10",
volume = "76",
number = "21",
pages = "7085--7092",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",

}

RIS

TY - JOUR

T1 - Lactococcal Abortive Infection Protein AbiV Interacts Directly with the Phage Protein SaV and Prevents Translation of Phage Proteins

A1 - Haaber,Jakob Brandt Borup

A1 - Samson,J.E.

A1 - Labrie,S.J.

A1 - Campanacci,V.

A1 - Cambillau,C.

A1 - Moineau,S.

A1 - Hammer,Karin

AU - Haaber,Jakob Brandt Borup

AU - Samson,J.E.

AU - Labrie,S.J.

AU - Campanacci,V.

AU - Cambillau,C.

AU - Moineau,S.

AU - Hammer,Karin

PB - American Society for Microbiology

PY - 2010

Y1 - 2010

N2 - AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein SaV. First, we showed that during phage infection of lactococcal AbiV(+) cells, AbiV rapidly inhibited protein synthesis. Among early phage transcripts, sav gene transcription was slightly inhibited while the SaV protein could not be detected. Analyses of other phage p2 mRNAs and proteins suggested that AbiV blocks the activation of late gene transcription, probably by a general inhibition of translation. Using size exclusion chromatography coupled with on-line static light scattering and refractometry, as well as fluorescence quenching experiments, we also demonstrated that both AbiV and SaV formed homodimers and that they strongly and specifically interact with each other to form a stable protein complex.

AB - AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein SaV. First, we showed that during phage infection of lactococcal AbiV(+) cells, AbiV rapidly inhibited protein synthesis. Among early phage transcripts, sav gene transcription was slightly inhibited while the SaV protein could not be detected. Analyses of other phage p2 mRNAs and proteins suggested that AbiV blocks the activation of late gene transcription, probably by a general inhibition of translation. Using size exclusion chromatography coupled with on-line static light scattering and refractometry, as well as fluorescence quenching experiments, we also demonstrated that both AbiV and SaV formed homodimers and that they strongly and specifically interact with each other to form a stable protein complex.

U2 - 10.1128/AEM.00093-10

DO - 10.1128/AEM.00093-10

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 21

VL - 76

SP - 7085

EP - 7092

ER -