Lab on a chip automates in vitro cell culturing

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Lab on a chip automates in vitro cell culturing. / Perozziello, Gerardo; Møllenbach, Jacob; Laursen, Steen; Di Fabrizio, Enzo; Gernaey, Krist; Krühne, Ulrich.

In: Microelectronic Engineering, Vol. 98, 2012, p. 655-658.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Author

Perozziello, Gerardo; Møllenbach, Jacob; Laursen, Steen; Di Fabrizio, Enzo; Gernaey, Krist; Krühne, Ulrich / Lab on a chip automates in vitro cell culturing.

In: Microelectronic Engineering, Vol. 98, 2012, p. 655-658.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{7743eecf152949199f7ee312b580d662,
title = "Lab on a chip automates in vitro cell culturing",
keywords = "Lab on a chip, Automated in vitro fertilization, Single cell cultivation, T and pH control, Rapid prototyping",
publisher = "Elsevier BV",
author = "Gerardo Perozziello and Jacob Møllenbach and Steen Laursen and {Di Fabrizio}, Enzo and Krist Gernaey and Ulrich Krühne",
year = "2012",
doi = "10.1016/j.mee.2012.07.027",
volume = "98",
pages = "655--658",
journal = "Microelectronic Engineering",
issn = "0167-9317",

}

RIS

TY - JOUR

T1 - Lab on a chip automates in vitro cell culturing

A1 - Perozziello,Gerardo

A1 - Møllenbach,Jacob

A1 - Laursen,Steen

A1 - Di Fabrizio,Enzo

A1 - Gernaey,Krist

A1 - Krühne,Ulrich

AU - Perozziello,Gerardo

AU - Møllenbach,Jacob

AU - Laursen,Steen

AU - Di Fabrizio,Enzo

AU - Gernaey,Krist

AU - Krühne,Ulrich

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.

AB - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.

KW - Lab on a chip

KW - Automated in vitro fertilization

KW - Single cell cultivation

KW - T and pH control

KW - Rapid prototyping

U2 - 10.1016/j.mee.2012.07.027

DO - 10.1016/j.mee.2012.07.027

JO - Microelectronic Engineering

JF - Microelectronic Engineering

SN - 0167-9317

VL - 98

SP - 655

EP - 658

ER -