• Author: Lykkeberg, Anne Kruse

  • Author: Sengeløv, Gitte

    Technical University of Denmark

  • Author: Cornett, Claus

  • Author: Tjørnelund, Jens

  • Author: Hansen, Steen Honoré

  • Author: Halling-Sørensen, Bent

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2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS. C-18 chromatographic column (100 mm x 19 turn W., 5 mum), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was deter-mined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.
Original languageEnglish
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number3
Pages (from-to)559-567
StatePublished - 2004
CitationsWeb of Science® Times Cited: No match on DOI


  • preparative HPLC, tandem mass spectrometry, resistance, microbial testing, NMR, 2-acetyl-2-decarboxamido-oxytetracycline
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ID: 3336066