Investigation of diversity of plasmids carrying the blaTEM-52 gene

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Investigation of diversity of plasmids carrying the blaTEM-52 gene. / Bielak, Eliza Maria; Bergenholtz, Rikke D.; Jørgensen, Mikael Skaanning; Sørensen, Søren J.; Hansen, Lars H.; Hasman, Henrik.

In: Journal of Antimicrobial Chemotherapy, Vol. 66, No. 11, 2011, p. 2465-2474.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Bielak, Eliza Maria; Bergenholtz, Rikke D.; Jørgensen, Mikael Skaanning; Sørensen, Søren J.; Hansen, Lars H.; Hasman, Henrik / Investigation of diversity of plasmids carrying the blaTEM-52 gene.

In: Journal of Antimicrobial Chemotherapy, Vol. 66, No. 11, 2011, p. 2465-2474.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{36fd10f0f11e4534ac8000ac311625ef,
title = "Investigation of diversity of plasmids carrying the blaTEM-52 gene",
keywords = "ESBLs, Human and non-human isolates, Antibiotic resistance",
publisher = "Oxford University Press",
author = "Bielak, {Eliza Maria} and Bergenholtz, {Rikke D.} and Jørgensen, {Mikael Skaanning} and Sørensen, {Søren J.} and Hansen, {Lars H.} and Henrik Hasman",
year = "2011",
doi = "10.1093/jac/dkr331",
volume = "66",
number = "11",
pages = "2465--2474",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",

}

RIS

TY - JOUR

T1 - Investigation of diversity of plasmids carrying the blaTEM-52 gene

A1 - Bielak,Eliza Maria

A1 - Bergenholtz,Rikke D.

A1 - Jørgensen,Mikael Skaanning

A1 - Sørensen,Søren J.

A1 - Hansen,Lars H.

A1 - Hasman,Henrik

AU - Bielak,Eliza Maria

AU - Bergenholtz,Rikke D.

AU - Jørgensen,Mikael Skaanning

AU - Sørensen,Søren J.

AU - Hansen,Lars H.

AU - Hasman,Henrik

PB - Oxford University Press

PY - 2011

Y1 - 2011

N2 - OBJECTIVES: To investigate the diversity of plasmids that carry blaTEM-52 genes among Escherichia coli and Salmonella enterica originating from animals, meat products and humans. METHODS: A collection of 22 blaTEM-52-encoding plasmids was characterized by restriction fragment length polymorphism (RFLP), replicon typing (by PCR or replicon sequencing), susceptibility testing, assessment of plasmid ability to self-transfer by conjugation and typing of the genetic environment of the blaTEM-52 gene. Detected IncI1 plasmids underwent further plasmid multilocus sequence typing. RESULTS: RFLP profiles demonstrated dissemination of blaTEM-52 in Denmark (imported meat from Germany), France, Belgium and the Netherlands from 2000 to 2006 by mainly two different plasmids, one encoding blaTEM-52b (IncX1A, 45 kb) and the other blaTEM-52c (IncI1, 80 kb). In addition, blaTEM-52b was also found to be located on various other plasmids belonging to IncA/C and IncL/M, while blaTEM-52c was found on IncN-like as well as on IncR plasmids. In the majority of cases (n = 21) the blaTEM-52 gene was located on a Tn3 transposon. Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transfer to a plasmid-free E. coli recipient. CONCLUSIONS: The blaTEM-52 gene found in humans could have been transmitted on transferable plasmids originating from animal sources. Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones. Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor blaTEM-1, the blaTEM-52 gene was not detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.

AB - OBJECTIVES: To investigate the diversity of plasmids that carry blaTEM-52 genes among Escherichia coli and Salmonella enterica originating from animals, meat products and humans. METHODS: A collection of 22 blaTEM-52-encoding plasmids was characterized by restriction fragment length polymorphism (RFLP), replicon typing (by PCR or replicon sequencing), susceptibility testing, assessment of plasmid ability to self-transfer by conjugation and typing of the genetic environment of the blaTEM-52 gene. Detected IncI1 plasmids underwent further plasmid multilocus sequence typing. RESULTS: RFLP profiles demonstrated dissemination of blaTEM-52 in Denmark (imported meat from Germany), France, Belgium and the Netherlands from 2000 to 2006 by mainly two different plasmids, one encoding blaTEM-52b (IncX1A, 45 kb) and the other blaTEM-52c (IncI1, 80 kb). In addition, blaTEM-52b was also found to be located on various other plasmids belonging to IncA/C and IncL/M, while blaTEM-52c was found on IncN-like as well as on IncR plasmids. In the majority of cases (n = 21) the blaTEM-52 gene was located on a Tn3 transposon. Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transfer to a plasmid-free E. coli recipient. CONCLUSIONS: The blaTEM-52 gene found in humans could have been transmitted on transferable plasmids originating from animal sources. Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones. Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor blaTEM-1, the blaTEM-52 gene was not detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.

KW - ESBLs

KW - Human and non-human isolates

KW - Antibiotic resistance

U2 - 10.1093/jac/dkr331

DO - 10.1093/jac/dkr331

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 11

VL - 66

SP - 2465

EP - 2474

ER -