• Author: Leifsson, Pall S.

    University of Copenhagen

  • Author: Iburg, Tine

    University of Copenhagen

  • Author: Jensen, Henrik E.

    University of Copenhagen

  • Author: Agerholm, Jørgen S.

    University of Copenhagen

  • Author: Kjelgaard-Hansen, Mads

    University of Copenhagen

  • Author: Wiinberg, Bo

    University of Copenhagen

  • Author: Heegaard, Peter M. H.

    Innate Immunology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870, Frederiksberg C, Denmark

  • Author: Astrup, Lærke B.

    University of Copenhagen

  • Author: Olsson, Anna E.

    University of Copenhagen

  • Author: Skov, Mette G.

    University of Copenhagen

  • Author: Aalbæk, Bent

    University of Copenhagen

  • Author: Nielsen, Ole L.

    University of Copenhagen

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Nine pigs were inoculated intravenously once or twice with 108 Staphylococcus aureus per kilogram body weight and sacrificed 12, 24 and 48 h after inoculation. Three sham-infected pigs served as controls. Blood samples were taken for bacteriology, haematology and clinical chemistry. A necropsy was carried out and tissue samples were collected for bacteriology and histology. The onset of clinical disease was seen at 7-8 h after inoculation. The blood bacterial counts remained low throughout the study. All infected pigs developed sepsis characterized by fever, neutrophilia, increased levels of C-reactive protein (CRP) and IL-6, and decreased levels of serum iron. The CRP and IL-6 levels peaked at 36 h, whereas IL-1 beta and tumour necrosis factor-alpha showed no obvious changes. Thromboelastography showed increasing hypercoagulability from 12 h and onwards, whereas the platelet numbers declined slightly throughout the experiment. The levels of serum aspartate aminotransferase and bilirubin were elevated at 24 and 36 h. In conclusion, sepsis and severe sepsis were induced as evidenced by dysfunction of the blood clotting system and the liver.
Original languageEnglish
JournalF E M S Microbiology Letters
Publication date2010
Volume309
Issue2
Pages208-216
ISSN0378-1097
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 14

Keywords

  • Clinical chemistry, Blood clotting, Severe sepsis, Liver, Staphylococcus aureus, Animal model
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