Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae. / Aabo, Thomas; Glückstad, Jesper; Siegumfeldt, Henrik; Arneborg, Nils.

In: Journal of the Royal Society. Interface, Vol. 8, No. 64, 2011, p. 1635-1643.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Aabo, Thomas; Glückstad, Jesper; Siegumfeldt, Henrik; Arneborg, Nils / Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae.

In: Journal of the Royal Society. Interface, Vol. 8, No. 64, 2011, p. 1635-1643.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{00e29b46c6a447f896447b085b12cc18,
title = "Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae",
keywords = "Permeability, Intracellular pH, Membrane, Yeast, Heat stress",
publisher = "The/Royal Society",
author = "Thomas Aabo and Jesper Glückstad and Henrik Siegumfeldt and Nils Arneborg",
year = "2011",
doi = "10.1098/rsif.2011.0148",
volume = "8",
number = "64",
pages = "1635--1643",
journal = "Journal of the Royal Society. Interface",
issn = "1742-5689",

}

RIS

TY - JOUR

T1 - Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae

A1 - Aabo,Thomas

A1 - Glückstad,Jesper

A1 - Siegumfeldt,Henrik

A1 - Arneborg,Nils

AU - Aabo,Thomas

AU - Glückstad,Jesper

AU - Siegumfeldt,Henrik

AU - Arneborg,Nils

PB - The/Royal Society

PY - 2011

Y1 - 2011

N2 - Internal pH regulation is vital for many cell functions, including transport mechanisms and metabolic enzyme activity. More specifically, transport mechanisms are to a wide degree governed by internal pH distributions. We introduce the term standard deviation of the intracellular pH (s.d.(pHint)) to describe the internal pH distributions. The cellular pH distributional response to external stress such as heat has not previously been determined. In this study, the intracellular pH (pHi) and the s.d.(pHint) of Saccharomyces cerevisiae cells exposed to supralethal temperatures were measured using fluorescence ratio imaging microscopy (FRIM). An exponential decline in pHi was observed after an initial small decline. For the first time, we report the use of FRIM for determining in vivo plasma membrane proton permeability coefficients in yeast. Furthermore, the exponential decay of pHi and the rupture of the cell plasma membrane, as measured by propidium iodide staining, at 70°C were not simultaneous but were separated by a significant temporal difference. Finally, a nonlinear relationship between the pHi and s.d.(pHint) was found; i.e. the s.d.(pHint) was significantly more sensitive to supralethal temperatures than pHi. s.d.(pHint) is therefore proposed as an early health/vitality indicator in S. cerevisiae cells exposed to heat stress.

AB - Internal pH regulation is vital for many cell functions, including transport mechanisms and metabolic enzyme activity. More specifically, transport mechanisms are to a wide degree governed by internal pH distributions. We introduce the term standard deviation of the intracellular pH (s.d.(pHint)) to describe the internal pH distributions. The cellular pH distributional response to external stress such as heat has not previously been determined. In this study, the intracellular pH (pHi) and the s.d.(pHint) of Saccharomyces cerevisiae cells exposed to supralethal temperatures were measured using fluorescence ratio imaging microscopy (FRIM). An exponential decline in pHi was observed after an initial small decline. For the first time, we report the use of FRIM for determining in vivo plasma membrane proton permeability coefficients in yeast. Furthermore, the exponential decay of pHi and the rupture of the cell plasma membrane, as measured by propidium iodide staining, at 70°C were not simultaneous but were separated by a significant temporal difference. Finally, a nonlinear relationship between the pHi and s.d.(pHint) was found; i.e. the s.d.(pHint) was significantly more sensitive to supralethal temperatures than pHi. s.d.(pHint) is therefore proposed as an early health/vitality indicator in S. cerevisiae cells exposed to heat stress.

KW - Permeability

KW - Intracellular pH

KW - Membrane

KW - Yeast

KW - Heat stress

UR - http://rsif.royalsocietypublishing.org/

U2 - 10.1098/rsif.2011.0148

DO - 10.1098/rsif.2011.0148

JO - Journal of the Royal Society. Interface

JF - Journal of the Royal Society. Interface

SN - 1742-5689

IS - 64

VL - 8

SP - 1635

EP - 1643

ER -