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Integration of an optical coherence tomography (OCT) system into an examination incubator to facilitate in vivo imaging of cardiovascular development in higher vertebrate embryos under stable physiological conditions. / Happel, Christoph M.; Thrane, Lars; Thommes, Jan; Männer, Jörg; Yelbuz, T. Mesud.

In: Annals of Anatomy, Vol. 193, No. 5, 2011, p. 425-435.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Happel, Christoph M.; Thrane, Lars; Thommes, Jan; Männer, Jörg; Yelbuz, T. Mesud / Integration of an optical coherence tomography (OCT) system into an examination incubator to facilitate in vivo imaging of cardiovascular development in higher vertebrate embryos under stable physiological conditions.

In: Annals of Anatomy, Vol. 193, No. 5, 2011, p. 425-435.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{47bbb1dc763b409c84b055b4f949bbe4,
title = "Integration of an optical coherence tomography (OCT) system into an examination incubator to facilitate in vivo imaging of cardiovascular development in higher vertebrate embryos under stable physiological conditions",
publisher = "Urban und Fischer Verlag",
author = "Happel, {Christoph M.} and Lars Thrane and Jan Thommes and Jörg Männer and Yelbuz, {T. Mesud}",
year = "2011",
doi = "10.1016/j.aanat.2011.04.006",
volume = "193",
number = "5",
pages = "425--435",
journal = "Annals of Anatomy",
issn = "0940-9602",

}

RIS

TY - JOUR

T1 - Integration of an optical coherence tomography (OCT) system into an examination incubator to facilitate in vivo imaging of cardiovascular development in higher vertebrate embryos under stable physiological conditions

A1 - Happel,Christoph M.

A1 - Thrane,Lars

A1 - Thommes,Jan

A1 - Männer,Jörg

A1 - Yelbuz,T. Mesud

AU - Happel,Christoph M.

AU - Thrane,Lars

AU - Thommes,Jan

AU - Männer,Jörg

AU - Yelbuz,T. Mesud

PB - Urban und Fischer Verlag

PY - 2011

Y1 - 2011

N2 - High-resolution in vivo imaging of higher vertebrate embryos over short or long time periods under constant physiological conditions is a technically challenging task for researchers working on cardiovascular development. In chick embryos, for example, various studies have shown that without appropriate maintenance of temperature, as one of the main environmental factors, the embryonic heart rate drops rapidly and often results in an increase in regurgitant flow. Hemodynamic parameters are critical stimuli for cardiovascular development that, for a correct evaluation of their developmental significance, should be documented under physiological conditions. However, previous studies were mostly carried out outside of an incubator or under suboptimal environmental conditions. Here we present, to the best of our knowledge, the first detailed description of an optical coherence tomography (OCT) system integrated into an examination incubator to facilitate real-time in vivo imaging of cardiovascular development under physiological environmental conditions. We demonstrate the suitability of this OCT examination incubator unit for use in cardiovascular development studies by examples of proof of principle experiments. We, furthermore, point out the need for use of examination incubators for physiological OCT examinations by documenting the effects of room climate (22 ◦C) on the performance of the cardiovascular system of chick embryos (HH-stages 16/17). Upon exposure to room climate, chick embryos showed a fast drop in the heart rate and striking changes in the cardiac contraction behaviour and the blood flow through the vitelline circulation. We have documented these changes for the first time by M-mode OCT and Doppler M-mode OCT.

AB - High-resolution in vivo imaging of higher vertebrate embryos over short or long time periods under constant physiological conditions is a technically challenging task for researchers working on cardiovascular development. In chick embryos, for example, various studies have shown that without appropriate maintenance of temperature, as one of the main environmental factors, the embryonic heart rate drops rapidly and often results in an increase in regurgitant flow. Hemodynamic parameters are critical stimuli for cardiovascular development that, for a correct evaluation of their developmental significance, should be documented under physiological conditions. However, previous studies were mostly carried out outside of an incubator or under suboptimal environmental conditions. Here we present, to the best of our knowledge, the first detailed description of an optical coherence tomography (OCT) system integrated into an examination incubator to facilitate real-time in vivo imaging of cardiovascular development under physiological environmental conditions. We demonstrate the suitability of this OCT examination incubator unit for use in cardiovascular development studies by examples of proof of principle experiments. We, furthermore, point out the need for use of examination incubators for physiological OCT examinations by documenting the effects of room climate (22 ◦C) on the performance of the cardiovascular system of chick embryos (HH-stages 16/17). Upon exposure to room climate, chick embryos showed a fast drop in the heart rate and striking changes in the cardiac contraction behaviour and the blood flow through the vitelline circulation. We have documented these changes for the first time by M-mode OCT and Doppler M-mode OCT.

KW - Optical coherence tomography

KW - Examination incubator

KW - Cardiovascular development

U2 - 10.1016/j.aanat.2011.04.006

DO - 10.1016/j.aanat.2011.04.006

JO - Annals of Anatomy

JF - Annals of Anatomy

SN - 0940-9602

IS - 5

VL - 193

SP - 425

EP - 435

ER -