Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
Original languageEnglish
JournalJournal of Micromechanics and Microengineering
Publication date2010
Volume20
Issue5
Pages55010
ISSN0960-1317
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 15
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