Injection molded chips with integrated conducting polymer electrodes for electroporation of cells
Publication: Research - peer-review › Journal article – Annual report year: 2010
We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
| Original language | English |
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| Journal | Journal of Micromechanics and Microengineering |
| Publication date | 2010 |
| Volume | 20 |
| Journal number | 5 |
| Pages | 55010 |
| ISSN | 0960-1317 |
| DOIs | |
| State | Published |
| Citations | Web of Science® Times Cited: 8 |
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ID: 5299754