Inflammatory and regenerative responses in salmonids following mechanical tissue damage and natural infection

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Standard

Inflammatory and regenerative responses in salmonids following mechanical tissue damage and natural infection. / Ingerslev, Hans-Christian; Lunder, Tor; Nielsen, Michael Engelbrecht.

In: Fish and Shellfish Immunology, Vol. 29, No. 3, 2010, p. 440-450.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Harvard

APA

CBE

MLA

Vancouver

Author

Ingerslev, Hans-Christian; Lunder, Tor; Nielsen, Michael Engelbrecht / Inflammatory and regenerative responses in salmonids following mechanical tissue damage and natural infection.

In: Fish and Shellfish Immunology, Vol. 29, No. 3, 2010, p. 440-450.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{35ceaa8a2adf4968a2a60bd4f08cb08b,
title = "Inflammatory and regenerative responses in salmonids following mechanical tissue damage and natural infection",
keywords = "Real-time PCR, Cytokine, Moritella viscosus, Tissue regeneration, Oncorhynchus mykiss, Toll-like receptors, Pathogen associated molecular patterns (PAMPS), Damage associated molecular patterns (DAMPs), Salmo salar, Inflammation",
publisher = "Academic Press",
author = "Hans-Christian Ingerslev and Tor Lunder and Nielsen, {Michael Engelbrecht}",
year = "2010",
doi = "10.1016/j.fsi.2010.05.002",
volume = "29",
number = "3",
pages = "440--450",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",

}

RIS

TY - JOUR

T1 - Inflammatory and regenerative responses in salmonids following mechanical tissue damage and natural infection

A1 - Ingerslev,Hans-Christian

A1 - Lunder,Tor

A1 - Nielsen,Michael Engelbrecht

AU - Ingerslev,Hans-Christian

AU - Lunder,Tor

AU - Nielsen,Michael Engelbrecht

PB - Academic Press

PY - 2010

Y1 - 2010

N2 - Locale responses in muscle tissue against either a sterile tissue damage or infection were compared in salmonid fish in order to examine the inflammatory responses and regeneration of tissue. From higher vertebrates both damage and infection are known to cause inflammation since DAMPs released from injured cells as well as PAMPs from the surface of pathogens are immunogenic. To examine this in salmonid fishes, Atlantic salmon (Salmo salar) were infected with Moritella viscosus, the causative agent of winter ulcer. Muscle tissue was sampled from infected fish at 4, 7 and 14 days post infection. Samples were obtained from site of lesions and from locations without clinical signs of disease and lesions. The tissue damage was performed in rainbow trout (Oncorhynchus mykiss) by applying sterile needles to skin and muscle tissue to one side of the fish. Samples were taken 7, 14, 21, 28 and 42 days post injury from the injured side and non-injured site (internal control). From both infected and damaged fish, samples were subject to real-time RT-PCR for measuring the expression of IL-1β, IL-8, IL-10, Hsp70, iNOS, TGF-β, TLR-5m, TLR-9, TLR-22, TGF-β, MMP-2, CTGF, myostatin-1αβ and collagen-1α which are coding for immunological factors and tissue regeneration. Locale, inflammatory responses were seen as strong up-regulation of IL-1β and IL-8 in both groups of fish, but it was more pronounced in infected fish. Expression of the toll-like receptors showed induction of TLR-5m following infection, but TLR-9 and TLR-22 following damage. Further, in both studies the regenerative genes TGF-β, MMP-2, CTGF, myostatin-1αβ were induced, but showed different kinetics. Collagen-1α was only induced in infected fish, probably due to heavier tissue damage in these.

AB - Locale responses in muscle tissue against either a sterile tissue damage or infection were compared in salmonid fish in order to examine the inflammatory responses and regeneration of tissue. From higher vertebrates both damage and infection are known to cause inflammation since DAMPs released from injured cells as well as PAMPs from the surface of pathogens are immunogenic. To examine this in salmonid fishes, Atlantic salmon (Salmo salar) were infected with Moritella viscosus, the causative agent of winter ulcer. Muscle tissue was sampled from infected fish at 4, 7 and 14 days post infection. Samples were obtained from site of lesions and from locations without clinical signs of disease and lesions. The tissue damage was performed in rainbow trout (Oncorhynchus mykiss) by applying sterile needles to skin and muscle tissue to one side of the fish. Samples were taken 7, 14, 21, 28 and 42 days post injury from the injured side and non-injured site (internal control). From both infected and damaged fish, samples were subject to real-time RT-PCR for measuring the expression of IL-1β, IL-8, IL-10, Hsp70, iNOS, TGF-β, TLR-5m, TLR-9, TLR-22, TGF-β, MMP-2, CTGF, myostatin-1αβ and collagen-1α which are coding for immunological factors and tissue regeneration. Locale, inflammatory responses were seen as strong up-regulation of IL-1β and IL-8 in both groups of fish, but it was more pronounced in infected fish. Expression of the toll-like receptors showed induction of TLR-5m following infection, but TLR-9 and TLR-22 following damage. Further, in both studies the regenerative genes TGF-β, MMP-2, CTGF, myostatin-1αβ were induced, but showed different kinetics. Collagen-1α was only induced in infected fish, probably due to heavier tissue damage in these.

KW - Real-time PCR

KW - Cytokine

KW - Moritella viscosus

KW - Tissue regeneration

KW - Oncorhynchus mykiss

KW - Toll-like receptors

KW - Pathogen associated molecular patterns (PAMPS)

KW - Damage associated molecular patterns (DAMPs)

KW - Salmo salar

KW - Inflammation

U2 - 10.1016/j.fsi.2010.05.002

DO - 10.1016/j.fsi.2010.05.002

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 3

VL - 29

SP - 440

EP - 450

ER -